Summary
During submerged cultivation of L-lysine producing Corynebacterium glutamicum 9366, oxygen limitation caused a three-fold increase in the intracellular pool of L-lysine while its excretion into the medium cased. At the same time, the phospholipid content of cells increased markedly whereas the proportion of oleic acid in the fatty acids of phospholipids decreased.
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References
Auzane, S. I. and Damberg, B. E. (1972) Microbial biomass and their metabolites. Zinatne, Riga, LSSR, 5–11
Babij, T., Moss, F. J. and Ralph, B. J. (1969) Biotechnol. Bioeng.11, 593–603
Demain, A. L. and Birnbaum, J. (1969) Curr. Top. Microbiol. Immunol. 46, 1–25
Fulco, A. J. (1972) J. Biol. Chem. 247, 3503–3510
Gräfe, U., Schada, W., Bocker, H. and Thrum, H. (1979). Z. Allg.Mikrobiol. 19, 721–726.
Hesse, G. and Geller, K. (1968) Mikrochimica Acta 3, 526–533
Hilliger, M. and Hänel, F. (1981) Biotechnology Letters 3, 219–224.
Kates, M. (1972) Techniques of lipidology. In: Laboratory Techniques in Biochemistry and Molecular Biology. Vol. 2, T. S. Work and E. Work (Eds.) Amsterdam, London.
Reczek, E. G. and Burton, D. N. (1979) Can. J. Microbiol. 25, 1239–1244
Shibukawa, M. and Ohsawa, T. (1966) Agric. Biol. Chem. 30, 750–758
Tosaka, O., Takinami, K. and Hirose, Y. (1978) Agric. Biol. Chem. 42, 745–752
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Hänel, F., Hilliger, M. & Gräfe, U. Effect of oxygen limitation on cellular L-lysine pool and lipid spectrum of Corynebacterium glutamicum. Biotechnol Lett 3, 461–464 (1981). https://doi.org/10.1007/BF00147554
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DOI: https://doi.org/10.1007/BF00147554