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Continuous production of patulin by immobilized cells ofPenicillium urticae in a stirred tank reactor

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Summary

Production of the mycotoxin, patulin, by immobilized cells ofPenicillium urticae was chosen as a model system to study the formation of secondary metabolites on a long term basis. Spores of the fungus were immobilized in carrageenan beads and allowed to germinate in situ by incubation in a growth medium. Production of the antibiotic by the populated biocatalyst was then followed in a continuous stirred tank reactor (CSTR) under three different regimes of nutrient feed. Using a nitrogen free feed, antibiotic production gradually declined but the cells could be re-activated by addition of a diluted growth medium. Prolonged production of patulin (up to 440 h) was observed when a source of nitrogen (ie. yeast extract) was supplied to the feed medium.

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Authors and Affiliations

  1. Division of Biochemistry, Department of Chemistry, University of Calgary, T2N 1N4, Calgary, Alberta

    A. Jones, B. H. Lesser & G. M. Gaucher

  2. Department of Chemical Engineering, University of Calgary, T2N 1N4, Calgary, Alberta

    D. Berk & L. A. Behie

Authors
  1. A. Jones
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  2. D. Berk
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  3. B. H. Lesser
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  4. L. A. Behie
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  5. G. M. Gaucher
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A publication of the University of Calgary Immobilized Cell Biotechnology Group.

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Jones, A., Berk, D., Lesser, B.H. et al. Continuous production of patulin by immobilized cells ofPenicillium urticae in a stirred tank reactor. Biotechnol Lett 5, 785–790 (1983). https://doi.org/10.1007/BF01386648

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  • DOI: https://doi.org/10.1007/BF01386648

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