Abstract
Variousin vitro techniques are available for plant propagation, including seed germination, micropropagation, meristem culture and callus culture. The role of these techniques in the conservation of endangered plants is discussed, using examples drawn from the work of the Micropropagation Unit at Kew.
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Beyl, C.A. and Sharma, G.C. (1983) Picloram induced somatic embryogenesis inGasteria andHaworthia.Plant Cell, Tiss. Org. Cult. 2, 123–32.
Bramwell, D. (1990) The role ofin vitro cultivation in the conservation of endangered species. InConservation Techniques in Botanic Gardens (J.E. Hernández Bermejo, M. Clemente and V. Heywood, eds.) pp. 3–15. Koenigstein, Germany: Koeltz Scientific Books.
Butcher, D. and Marlow, S.A. (1989) Asymbiotic germination of epiphytic and terrestrial orchids. InModern methods in orchid conservation: the role of physiology, ecology and management (H.W. Pritchard, ed.) pp. 31–8, Cambridge: Cambridge Univ. Press.
Clements, M.A., Muir, H.J. and Cribb, P.J. (1986) Preliminary report on the symbiotic germination of European orchids.Kew Bull. 41, 437–45.
Cronk, Q.C.B. (1983) The decline of the redwoodTrochetiopsis erythroxylon on St. Helena.Biol. Conserv. 26, 163–74.
Cronk, Q.C.B. (1986) The decline of the St Helena ebonyTrochetiopsis melanoxylon.Biol. Conserv. 35, 159–72.
Davis, S.D., Droop, S.J.M., Gregerson, P., Henson, L., Leon, C. J., Lamlein Villa-Lobos, J., Synge, H., and Zantovska, J. (1986)Plants in Danger. What do we know? International Union for the Conservation of Nature and Natural Resources. Gland, Switzerland and Cambridge, UK.
Debergh, P.C. and Zimmerman, R.H. (eds.) (1991) Micropropagation: technology and application. Kluwer Academic Publications.
Dodds, J.H. (ed.) (1991) In vitromethods for conservation of plant genetic resources. London: Chapman and Hall.
Falk, D.A. (1990) Integrated strategies for conserving plant genetic diversity.Ann. Missouri Bot. Gard. 77, 38–47.
Fay, M.F. (1988) Micropropagation at the Royal Botanic Gardens, Kew.Bot. Gard. Conserv. News 1 (3), 42–5.
Fay, M.F. (1992a) Conservation of rare and endangered plants usingin vitro methods. In VitroCell Dev. Biol. 28P, 1–4.
Fay, M.F. (1992b) Weaning micropropagated plantelts.Bot. Gard. Conserv. News 1(10), 41–43.
Fay, M.F. (1994) Micropropagation of plants from the Canary Islands.Bol. Mus. Mun. Funchal. (In press).
Fay, M.F. and Gratton, J. (1992), Tissue culture of cacti and other succulents: a literature review and a report of micropropagation at Kew.Bradleya 10, 33–48.
Fay, M.F. and Muir, H.J. (1990) The role of micropropagation in the conservation of European plants. InConservation Techniques in Botanic Gardens (J.E. Hernández Bermejo, M. Clemente and V. Heywood, eds.) pp. 27–32. Koenigstein, Germany: Koeltz Scientific Books.
Fay, M.F. and Redwood, G.N. (1990) Abstract VIIth International Congress on Plant Tissue and Cell Culture,99.
George, E. F., Puttock, D.J.M. and George, H.J. (1987)Plant culture media. Vol. 1. Formulations and uses. Exegetics Ltd. Basingstoke, UK.
George, E.F., Puttock, D.J.M. and George, H.J. (1988)Plant culture media. Vol. 2. Commentary and analysis. Exegetics Ltd. Basingstoke, UK.
George, E.F. and Sherrington, P.D. (1984)Plant propagation by tissue culture. Handbook and directory of commercial laboratories. Exegetics Ltd.
Gratton, J. and Fay, M.F. (1990) Vegetative propagation of cacti and other succulentsin vitro. InMethods in Molecular Biology. Vol. 6. Plant Cell and Tissue Culture (J.W. Pollard and J.M. Walker (eds.) pp. 219–25. New Jersey, USA. Humana Press.
Hadley, G. (1982) Orchid mycorrhiza. InOrchid Biology: Reviews and Perspectives, II (J. Arditti, ed.) pp. 83–118. Ithaca and London Cornell Univ. Press.
Harley, J.L. and Smith, S.E. (1983)Mycorrhizal symbiosis. London: Academic Press.
Jacobsen, H.-J. and Dohmen, G. (1990) Modern plant biotechnology as a tool for the reestablishment of genetic variability inSophora toromiro.Courier Forsch.-Inst. Senckenberg 125, 233–37.
Knudson, L. (1946) A new nutrient solution for the germination of orchid seed.Am. Orchid Soc. Bull. 15, 214–7.
Light, M.H.S. and MacConaill, M. (1990) Characterization of the optimal capsule development stage for embryo culture ofCypripedium calceolus var.pubescens. InNorth American Native Terrestrial Orchid Propagation Conf. Proc. (C.E. Sawyers, ed.) pp. 92, Pennsylvania: Brandywine Conservancy.
Maunder, M. (1992) Plant reintroduction: an overview.Biodiver. Conserv. 1, 51–61.
Melliss, J.C. (1875)St. Helena: a physical, historical, and topographical description of the island, including its geology, fauna, flora, and meteorology. London: L. Reeve and Co.,
Mistretta, O., Rieseberg, L.H. and Elias, T.S. (1991) Botanic gardens and the preservation of biological diversity.Evol. Trends Plants 5 19–22.
Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures.Physiol. Plant. 15, 473–97.
Muir, H.J. (1989) Germination and mycorrhizal fungus compatibility in European orchids. InModern methods in orchid conservation: the role of physiology, ecology and management (H.W. Pritchard, ed.) pp. 39–56, Cambridge: Cambridge Univ. Press.
Pence, V. C. (1990)Abstracts VIIth International Congress on Plant Tissue and Cell Culture, 377.
Ramsay, R.R., Dixon, K.W. and Sivasithamparam, K. (1989) Patterns of infection and endophytes associated with Western Australian orchids.Lindleyana 1, 203–14.
Redwood, G.N. and Bowling, J.C. (1990) Micropropagation ofNepenthes species.Bot. Gard. Microprop. News 1, 19–20.
Synge, H. (1991) Conserving the wild plants of Gran Canaria. Cabildo Insular de Gran Canaria.
Torelli, G. and Severini, C. (1991) Asymbiotic germination ofCypripedium calceolus L. In9th European Orchid Congress Proceedings Rome: Orto Botanico
Vacin, E.F. and Went, F.W. (1949) Some pH changes in nutrient solutions.Bot. Gaz. 110, 605–13.
Villalobos, V. M., Ferreira, P. and Mora, A. (1991), The use of biotechnology in the conservation of tropical germplasm.Biotech. Adv. 9, 197–215.
Warcup, J.H. (1973) Symbiotic germination of some Australian terrestrial orchids.New Phytol. 72, 387–92.
Wilkins, C.P., Bengochea, T. and Dodds, J.H. (1982) The use ofin vitro methods for plant genetic conservation.Outlook on Agr. 11, 67–72.
Withers, L.A., Wheelans, S.K. and Williams, J.T. (1990)In vitro conservation of crop germplasm and the IBPGR databases.Euphytica 45, 9–22.
Woods A. (1985) The potential for thein vitro propagation of a number of economically important species for arid regions. InPlants for Arid Lands. (G.E. Wickens, J.R. Goodin and D.V. Field), pp. 333–342. Royal Botanic Gardens, Kew.
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Fay, M.F. In what situations isin vitro culture appropriate to plant conservations?. Biodivers Conserv 3, 176–183 (1994). https://doi.org/10.1007/BF02291887
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DOI: https://doi.org/10.1007/BF02291887