Biologia plantarum 39:349-353, 1997 | DOI: 10.1023/A:1001015806870
Phenylalanine ammonia-lyase activity in alfalfa suspension cultures treated with conidia and elicitors of Verticillium albo-atrum
- 1 Department of Agro-Environmental Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
Alfalfa (Medicago sativa L.) cell suspension cultures of Verticillium albo-atrum resistant and susceptible genotypes were established from leaf callus tissues. Treatment of cultures with conidia and heat-released elicitors of V. albo-atrum induced a large increase in the activity of phenylalanine ammonia-lyase, only in the cells of the resistant genotypes with a maximum after 12 h. In co-cultivation with the fungal conidia and resistant cell lines, the production of spores were inhibited.
Keywords: Medicago sativa; resistant and susceptible genotypes
Prepublished online: April 1, 1997; Published: November 1, 1997 Show citation
References
- Bolwell, G.P., Bell, J.N., Craamer, C.L., Schuch, W., Lamb, C.J., Dixon, R.A.: L-phenylalanine ammonia-lyase from Phaseolus vulgaris. Characterization and differential induction of multiple forms from elicitor-treated cell suspension cultures.-Eur. J. Biochem. 149: 411-419, 1985. Go to original source...
- Buiatti, M., Scala, A., Bettini, P., Nascaari, G., Morpurgo, R., Bogoni, P., Pellegrini, G., Gemelli, F., Venturo, R.: Correlation between in vivo resistance to Fusarium and in vitro response to fungal elicitor and toxic substances in carnation.-Theor. appl. Genet. 70: 42-47, 1985. Go to original source...
- Hahlbrock, K., Schell, D.: Physiology and molecular biology of phenylpropanoid metabolism.-Annu. Rev. Plant Physiol. Plant mol. Biol. 40: 347-369, 1989. Go to original source...
- Koike, M., Nanbu, K., Shimada, T.: Alfalfa-Verticillium albo-atrum interaction: In vitro responses of alfalfa callus to fungal culture filtrates and cell wall components.-J. Jap. Grassland Sci. 37: 412-419, 1992.
- Koike, M., Shimada, T.: Alfalfa-Verticillium albo-atrum interaction. II. In vitro peroxidase and phenylalanine ammonia-lyase activities enhanced by treatment with fungal elicitor.-Plant Tissue Cult. Lett. 9: 81-85, 1992. Go to original source...
- Koike, M., Yoshida, Y., Kagaya, Y., Shimada, T.: In vitro selection and somaclonal variation in alfalfa Verticillium wilt.-Plant Tissue Cult. Lett. 8: 152-157, 1991. Go to original source...
- Lamb, C.J., Lawton, M.A., Dron, M., Dixon, R.A.: Signal and transduction mechanisms for activation of plant defense against microbial attack.-Cell 56: 215-224, 1989. Go to original source...
- Latunde-Dada, A.O., Lucas, J.A.: Influence of temperature on host resistance and fungal sensitivity to medicarpin in lucerne callus lines infected with Verticillium albo-atrum.-Physiol. mol. Plant Pathol. 28: 89-97, 1986. Go to original source...
- Latunde-Dada, A.O., Dixon, R.A., Lucas, J.A.: Induction of phytoalexin biosynthetic enzymes in resistant and susceptible lucerne callus lines infected with Verticillium albo-atrum.-Physiol. mol. Plant Pathol. 31: 15-23, 1987. Go to original source...
- Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951. Go to original source...
- Shenk, R.U., Hilderbrandt, A.C.: Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell culture.-Can. J. Bot. 50: 199-204, 1972. Go to original source...