Abstract
Injection of 1×106 CC531 colonic carcinoma cells into the mesenteric lymph nodes of Wag/Rij rats resulted in the growth of tumors within the lymph nodes. These were apparent after 3 days, whereas lung and liver metastases were not observed until 5 weeks after inoculation.In vivo labeling with bromodeoxyuridine (BrdU) followed by immunostaining with an anti-BrdU monoclonal antibody showed a marked difference in the proportion of labeled cells of the metastases at different times after inoculation: after 3 days, many tumor cells but also many stromal cells were labeled; after 7 and 11 days, however, far less stromal cells were positive, most labeled cells being tumor cells.
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Nagel, J.D., Mooi, W.J. & McVie, J.G. New, simple model of mesenteric lymph node metastases in the rat. Clin Exp Metast 7, 453–459 (1989). https://doi.org/10.1007/BF01753665
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DOI: https://doi.org/10.1007/BF01753665