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Specific epstein-barr virus serological response in patients with nasopiiaryngeal carcinoma detected by immunoblotting

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Abstract

The specific humoral immune response against Epstein-Barr virus (EBV) antigens in patients with nasopharyngeal carcinoma (NPC) was compared to that of patients with infectious mononucleosis (IM) and other EBV-seropositive subjects using immunoblotting technique. Almost all sera from E,BV serologically associated NPC reacted reproducibly with a major group of polypeptides (four to six) of early antigen (EA) complex with molecular weights ranging from 50 kD to 58 kD, and with some additional polypeptides. Sera from IM-patients reproducibly recognized only one polypeptide of 50 kD belonging to the major group of polypeptides of EA-complex. Sera from patients with other types of head and neck cancer and relatively high levels of IgG antibody against viral capsid antigen (VCA) and EA did not react reproducibly with any of the EBV-associated proteins.

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References

  1. AlldayM.J., McGillivrayA.J. (1985): Epstein-Barr virus nuclear antigen (EBNA): size polymorphism of EBNA1. - J. Gen. Virol. 66: 1595–1600.

    Google Scholar 

  2. BatteigerB., NewhallJ., JonesR.B. (1982): The use of tween-20 as blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. - J. Immunol. Methods 55: 297–307.

    Google Scholar 

  3. BodemerW.W., SummersW.C., NiedermanJ.C. (1980): Detection of virus-specific antigens in EB-(P3HRl) virus-superinfected Raji cells by immunoprecipitation.- Virology 103: 340–349.

    Google Scholar 

  4. CeveniniR., DonatiM., RumpianesiF. (1982): Primary Epstein-Barr virus infection in children with malignant disorders. Effect of therapy on EBV antibodies. - J. Infection 5: 73–79.

    Google Scholar 

  5. CeveniniR., DonatiM., RumpianesiF., MoroniA., PaolucciP. (1984): An immunoperoxidase assay for the detection of specific IgA antibody in Epstein-Barr virus inflections. - J. Clin. Pathol. 37: 440–443.

    Google Scholar 

  6. CeveniniR., DonatiM., CalicetiU., MoroniA., TambaI., RumpianesiF. (1986): Evaluation of antibodies to Epstein-Barr virus in Italian patients with nasopharyngeal carcinoma. - J. Infection 12: 127–131.

    Google Scholar 

  7. DeSchryverA., FribergS., KleinG., HenleW., HenleG., DeThèG., CliffordP., HoH.C. (1969): Epstein-Barr virus associated antibody patterns in carcinoma of post-nasal space. - Clin. Experimen. Immunol. 5: 443–459.

    Google Scholar 

  8. DolkenG., HechtT., WeitzmannU., WagnerM., LohrG., HirschF. (1986): Purification of a polypeptide complex (p52) belonging to the D-subspecificities of Epstein-Barr virus-induced early antigens. - Virology 148: 58–73.

    Google Scholar 

  9. GazzoloL., DeThèG., VuillaumeM., HoH.C. (1972): Nasopharyngeal carcinoma II. Ultrastructure of normal mucosa, tumor biopsies, and subsequent epithelial growth in vitro. - J. Nat. Cancer Inst. 48: 73–86.

    Google Scholar 

  10. HadarT., RahimaM., KahanE., SidiJ., RakowskiE., SarovB., SarovI. (1986): Significance of specific Epstein-.Barr virus IgA and elevated IgG antibodies to viral capsid antigens in nasopharyngeal carcinoma patients. - J. Med. Virol. 20: 329–339.

    Google Scholar 

  11. HenleG., HenleW., KleinG. (1971): Demonstration of two distinct components in the early antigen complex of Epstein-Barr virus infected cells. - Int. J. Cancer 8: 272–283.

    Google Scholar 

  12. HenleG., HenleW. (1976): Epstein-Barr virus-specific serum antibodies as outstanding feature of nasopharyngeal carcinoma. - Int. J. Cancer 17: 1–7.

    Google Scholar 

  13. KallinB., LukaJ., KleinG. (1979): Immunochemical characterization of Epstein-Barr virus-associated early and late antigens in n-butyrate-treated P3HR1 cells. - J. Virol. 32: 710–716.

    Google Scholar 

  14. KleinG. (1979): The relationship of the virus to nasopharyngeal carcinoma. In: Epstein M.A., Achong B.G.(eds) ≪ The Epstein-Barr virus ≫. Springer-Verlag, Berlin, Heidelberg, New York. pp. 339–350.

    Google Scholar 

  15. KruegerG.R.F., WustrowJ. (1981): Current histological classification of nasopharyngeal carcinoma (NPC) at Cologne University. In: Grundman E., Krueger G.R.F., Ablashi D.V. (eds): Cancer Campaign: nasopharyngeal carcinoma. Gustav Fisher Verlag, Stuttgart, New York. pp. 11–15.

    Google Scholar 

  16. LaemmliU.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. - Nature 227: 680–685.

    Google Scholar 

  17. LunJ.C., ChoiE.I., PaganoJ. (1985): Qualitative and quantitative analyses of Epstein-Barr virus early antigen diffuse component by blotting enzyme-linked immunosorbent assay with a monoclonal antibody. - J. Virol. 53: 793–799.

    Google Scholar 

  18. OldL., BoyseE.A., OettgenH.F., deHarvenE., GeeringG., WilliamsonB. (1966): Precipitating antibody in human serum to an antigen present in cultured Burkitt's lymphoma cells. - P.N.A.S. 56: 1699–1704.

    Google Scholar 

  19. PearsonG.R., WilandL.F., BryanN.H. (1981): Evaluation of antibodies to Epstein-Barr virus (EBV) in the diagnosis of American nasopharyngeal carcinoma. In: Grundman E., Krueger G.R.F., Ablashi D.V. (eds.): Cancer Campaign: nasopharyngeal carcinoma. Gustav Fisher Verlag. Berlin, Stuttgart, New York. pp. 231–236.

    Google Scholar 

  20. PearsonG.R., VromanB., ChaseB., SculleyT., HummelM., KiefE. (1983): Identification of polypeptide components of Epstein-Barr virus early antigen complex with monoclonal antibodies. -J. Virol. 47: 193–201.

    Google Scholar 

  21. ReedmanB.M., KleinG. (1973): Cellular localization of an Epstein,Barr virus (EBV);associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines. - Int. J. Cancer 11: 499–520.

    Google Scholar 

  22. SculleyT.B., WalkerP.J., MossD.J., PopeJ. (1984): Identification of multiple Epstein-Barr virus-induced nuclear antigen with sera from patients with rheumathoid arthritis. - J. Virol. 52: 88–93.

    Google Scholar 

  23. SculleyT.B., SculleyD.G., PopeJ.H. (1985): Identification of Epstein-Barr virus-induced polypeptides in P3HR1-cells by protein immunoblot. -J. Gen. Virol. 66: 1113–1122.

    Google Scholar 

  24. StrnadB., SchusterT., HopkinsR.F., Neubauer R.H., RabinH. (1981): Identification of an Epstein-Barr virus nuclear antigen by fluoro-immuno electrophoresis and radioimmunoelectrophoresis. -J. Virol. 38: 996–1004.

    Google Scholar 

  25. TowbinH., StaehelinT., GordonJ. (1979): Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. - P.N.A.S. 76: 4350–4354.

    Google Scholar 

  26. YammamotoN., Zur HausenH. (1980): Effect of inhibition of DNA synthesis on Epstein-Barr virus induction by tumor promoters. - Virology 101: 104–110.

    Google Scholar 

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Authors and Affiliations

  1. Istituto di Microbiologia, Università di Bologna, Ospedale S. Orsola, Via Massarenti 9, 40138, Bologna, Italy

    R. Cevenini, M. Donati, A. Moroni & M. La Placa

  2. Istituto di Otorinolaringologia, Università di Bologna, Ospedale S. Orsola, Via Massarenti 9, 40138, Bologna, Italy

    U. Caliceti & A. Rinaldi-Ceroni

Authors
  1. R. Cevenini
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  2. M. Donati
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  3. A. Moroni
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  4. U. Caliceti
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  5. A. Rinaldi-Ceroni
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  6. M. La Placa
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Cevenini, R., Donati, M., Moroni, A. et al. Specific epstein-barr virus serological response in patients with nasopiiaryngeal carcinoma detected by immunoblotting. Eur J Epidemiol 4, 301–305 (1988). https://doi.org/10.1007/BF00148913

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