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A new 2D NMR method for measurement of JHH coupling constants

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Summary

A new 2D NMR pulse sequence for E.COSY-type measurement of JHH coupling constants is introduced. It exploits a heteronuclear spin, e.g., 13C, for displacement in the ω1 frequency dimension via a large heteronuclear J coupling. The experiment is demonstrated by application to a heptapeptide at the natural abundance 13C level. It is suitable, for example, for measurement of 3JHH and 4JHH coupling constants in peptides and proteins.

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Authors and Affiliations

  1. Department of Chemistry, University of Copenhagen, The H.C. Ørsted Institute, Universitetsparken 5, DK-2100, Copenhagen Ø, Denmark

    Morten Dahl Sørensen & Jens J. Led

  2. Novo Nordisk, Novo Allé, DK-2880, Bagsværd, Denmark

    Ole Winneche Sørensen

Authors
  1. Morten Dahl Sørensen
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  2. Jens J. Led
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  3. Ole Winneche Sørensen
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Sørensen, M.D., Led, J.J. & Sørensen, O.W. A new 2D NMR method for measurement of JHH coupling constants. J Biomol NMR 4, 135–141 (1994). https://doi.org/10.1007/BF00178342

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  • DOI: https://doi.org/10.1007/BF00178342

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