Abstract
An enzyme-linked immunosorbent assay method for serodiagnosis of cancers was developed by employing histone H2B. This method measures anti-histone H2B antibody levels in sera and includes a device for coating the plastic immunoplate with a mixture of histone H2B and diluted fetal calf serum. The coating of immunoplates with this mixture decreased apparent sensitivity of the assay compared with that in the absence of fetal calf serum, but effective reduction of nonspecific background enabled a specific assay of anti-histone H2B antibody with excellent reproducibility. By this method cancer patients were discriminated from normal healthy subjects at detection rates of 37% for lung cancer, 33% for liver cancer, 50% for pancreatic cancer, 42% for colon cancer, and 78% for cervical cancer. However, stomach and esophagus cancers showed detection rates of less than 17%, which are comparable to the values for benign diseases. It is likely that this assay method detects squamous cell carcinomas at relatively high rates.
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Abbreviations
- ELISA:
-
enzyme-linked immunosorbent assay
- FCS:
-
fetal calf serum
- PBS:
-
phosphate buffered saline
- BSA:
-
bovine serum albumin
- PBS-Tween:
-
phosphate-buffered saline containing 0.05% Tween 20
- PBS-BSA-Tween:
-
phosphate-buffered saline containing 1 mg/ml bovine serum albumin and 0.05% Tween 20
- ABTS:
-
2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt;
- S.D.:
-
standard deviation
- CEA:
-
carcinoembryonic antigen
- SCC:
-
squamous cell carcinoma-related antigen
- NSE:
-
neuron-specific enolase
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Kamei, M., Kato, M., Mochizuki, K. et al. Serodiagnosis of cancers by ELISA of anti-histone H2B antibody. Biotherapy 4, 17–22 (1992). https://doi.org/10.1007/BF02171705
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DOI: https://doi.org/10.1007/BF02171705