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Simple and non-radioactive method for determination of sialyltransferase activity

  • Published:
Biotechnology Techniques

Abstract

A new, non-radioactive and cheap colorimetric method for determination of activity of sialyltransferases of various specifities using natural substrates based on 2-thiobarbituric acid assay is presented. The assay was tested with three different sialyltransferases (a-2,3 and a-2,6) and compared with the radioactive assay.

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Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Víde nska 1083, CZ 142 20, Prague 4, Czech Republic

    Vladimír Kùren

  2. Institute of Organic Chemistry, University of Hamburg, Martin-Luther-King Platz 6, D-20146, Hamburg, Germany

    Joachim Thiem

Authors
  1. Vladimír Kùren
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  2. Joachim Thiem
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Kùren, V., Thiem, J. Simple and non-radioactive method for determination of sialyltransferase activity. Biotechnology Techniques 11, 323–326 (1997). https://doi.org/10.1023/A:1018423513138

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  • DOI: https://doi.org/10.1023/A:1018423513138

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