Abstract
Saccharomyces cerevisiae lacks most of the depolymerising enzymes required for efficient hydrolysis and utilisation of polysaccharide-rich biomass. To allow extracellular degradation of polysaccharides, genes encoding amylopullulanase (LKA1), pectate lyase (PEL5), polygalacturonase (PEH1), endo-β-1,4-D-glucanase (END1), cellobiohydrolase (CBH1), exo-β-1,3-D-glucanase (EXG1), cellobiase (β-glucosidase; BGL1) and endo-β-D-xylanase (XYN4) were introduced jointly into a laboratory strain of S. cerevisiae. These transformants were able to grow on starch, pectate, cellobiose and to some extent on cellulose (solka-floc and lichenan). These results pave the way for the development of one-step bioconversion processing of plant biomass in the fuel, animal feed, baking and beverage industries. © Rapid Science Ltd. 1998
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allen, J.B. and Elledge, S.J. (1994). Yeast 10, 1267-1272.
Biely, P. (1993). Biochemical aspects of the production of microbial hemicellulases. In: Hemicellulose and hemicellulases, M.P. Coughlan and G.P. Hazlewood, eds. pp. 29-52, London: Portland Press Ltd.
Coughlan, M.P. (1990). Cellulose degradation by fungi. In: Microbial enzymes and biotechnology, W.M. Fogarty and C.T. Kelley eds. pp. 1-36, London: Elsevier Applied Science.
Crous, J.M., Van Zyl, W.H. and Pretorius, I.S. (1995.) Curr. Genet. 28, 467-473.
Crous, J.M., Pretorius, I.S. and Van Zyl, W.H. (1996). Appl. Microbiol. Biotechnol. 46, 256-260.
D'Aguanno, V.S. and Pretorius, I.S. (1994.) Biotechnol. Lett. 16, 727-732.
Hill, J., Ian, K.A., Donald, G. and Griffiths, D.E. (1991). Nucleic Acids Res. 19, 5791.
Janse, B.J.H. and Pretorius, I.S. (1993.) Curr. Genet. 24, 32-37.
Janse, B.J.H. and Pretorius, I.S. (1995.) Appl. Microbiol. Biotechnol. 42, 878-883.
La Grange, D.C., Pretorius, I.S. and Van Zyl, W.H. (1996). Appl. Environ. Microbiol. 62, 1036-1044.
La Grange, D.C., Pretorius, I.S. and Van Zyl, W.H. (1997). Appl. Microbiol. Biotechnol. 47, 262-266.
Laing, E. and Pretorius, I.S. (1992). Gene 121, 35-45.
Laing, E. and Pretorius, I.S. (1993a). J. Appl. Bacteriol. 75, 149-158.
Laing, E. and Pretorius, I.S. (1993b). Appl. Microbiol. Biotechnol. 39, 181-188.
Lynd, L. R. (1996). Ann. Rev. Energy Environ. 21:403-465.
Luttig, M., Pretorius, I.S. and Van Zyl, W.H. (1997). Biotechnol. Lett. 19, 411-415.
Moes, C.J., Pretorius I.S. and Van Zyl, W.H. (1996.) Biotechnol. Lett. 18, 269-27.
Pretorius, I.S. (1997). Utilization of polysaccharides by Saccharomyces cerevisiae. In: Yeast sugar metabolism, F.K. Zimmerman and K.-D. Entian eds. pp. 459-500. Lancaster: Technomic Publishing Company Inc.
Pretorius, I.S., Chow, T., Modena, D. and Marmur, J. (1986). Mol. Gen. Genet. 203, 29-35.
Pretorius, I.S., Laing, E., Pretorius, G.H.J. and J. Marmur. (1988.) Curr. Genet. 13, 9-13.
Sambrook, J., Fritch, E.F. and Miniatis, T. (1989). Molecular cloning. A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York: Cold Spring Harbor.
Steyn, A.J.C. and Pretorius, I.S. 1991. Gene 100, 85-93.
Steyn, A.J.C. and Pretorius, I.S. (1995). Curr. Genet. 28, 526-533.
Steyn, A.J.C., Marmur, J. and Pretorius, I.S. (1995). Gene 166, 65-71.
Steyn, A.J.C., Marmur, J. and Pretorius, I.S. (1996). Yeast 12: 925-937.
Van Rensburg, P., Van Zyl, W.H. and Pretorius, I.S. (1994). Curr. Genet. 27, 17-22.
Van Rensburg, P., Van Zyl, W.H. and Pretorius, I.S. (1995). Biotechnol. Lett. 17, 481-486.
Van Rensburg, P., Van Zyl, W.H. and Pretorius, I.S. (1996). Curr. Genet. 30, 246-250.
Van Rensburg, P., Van Zyl, W.H. and Pretorius, I.S. (1997). J. Biotechnol. 55, 43-53.
Van Rensburg, P., Van Zyl, W.H. and Pretorius, I.S. (1998). Yeast 14, 67-76.
Vihinen, M. and Mäntsälä, P. (1989). Crit. Rev. Biochem. Mol. Biol. 24, 329-418.
Whitaker, J.R. (1990). Microbial pectolytic enzymes. In: Microbial enzymes and biotechnology, W.M. Fogarty and C.T. Kelley eds. pp. 133-176, London: Elsevier Applied Science.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Petersen, S., van Zyl, W. & Pretorius, I. Development of a polysacharide degrading strain of Saccharomyces cerevisiae. Biotechnology Techniques 12, 615–619 (1998). https://doi.org/10.1023/A:1008829129516
Issue Date:
DOI: https://doi.org/10.1023/A:1008829129516