Abstract
α-Chymotrypsin and poly(ethylene glycol) were mixed in an aqueous phosphate buffer solution and lyophilized. The preparation was readily soluble in anhydrous isooctane. The polymer/enzyme complex, i.e., poly(ethylene glycol)/α-chymotrypsin, was catalytically active in anhydrous isooctane even when the molecular ratio of them was unity.
Similar content being viewed by others
References
Blinkovsky AM, Khmelnitsky YL, Dordick JS (1994) Organosoluble enzyme-polymer complexes: A novel type of biocatalyst for nonaqueous media. Biotechnol. Tech. 8: 33-38.
Gupta MN (1992) Enzyme function in organic solvents. Eur. J. Biochem. 203: 25-32.
Inada Y, Matsushima A, Hiroto M, Nishimura H, Kodera Y (1994) Modification of proteins with polyethylene glycol derivatives. Methods Enzymol. 242: 65-90.
Klibanov AM (1989) Enzymatic catalysis in anhydrous organic solvents. Trends Biochem. Sci. 14: 141-144.
Klibanov AM (1997) Why are enzymes less active in organic solvents than in water? Trends Biotechnol. 15: 97-101.
Mabrouk PA (1997) The use of poly(ethylene glycol)-enzymes in nonaqueous enzymology. ACS Symp. Ser. 680: 118-133.
Okahata Y, Fujimoto Y, Ijiro K (1995) A lipid-coated lipase as an enantioselective ester synthesis catalyst in homogeneous organic solvents. J. Org. Chem. 60: 2244-2250.
Okazaki S, Kamiya N, Goto M (1997) Application of novel preparation method for surfactant-protease complexes catalytically active in organic media. Biotechnol. Prog. 13: 551-556.
Otamiri M, Adlercreutz P, Mattiasson B (1992) Complex formation between chymotrypsin and ethyl cellulose as a means to solubilize the enzyme in active form in toluene. Biocatalysis 6: 291-305.
Otamiri M, Adlercreutz P, Mattiasson B (1994) Polymer-polymer organic solvent two-phase system: A new type of reaction medium for bioorganic synthesis. Biotechnol. Bioeng. 43: 987-994.
Sugimura Y, Fukunaga K, Matsuno T, Nakao K, Goto M, Nakashio F (1998) A study on the lipid-coating of lipases. Biochem. Eng. J. 2: 137-143.
Vakurov AV, Gladilin AK, Levashov AV, Khmelnitsky YL (1994) Dry enzyme-polymer complexes: Stable organosoluble biocatalysts for nonaqueous enzymology. Biotechnol. Lett. 16: 175-178.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Murakami, Y., Hirata, A. Complex between α-Chymotrypsin and poly(ethylene glycol) catalytically active in organic media. Biotechnology Techniques 13, 545–548 (1999). https://doi.org/10.1023/A:1008987431361
Issue Date:
DOI: https://doi.org/10.1023/A:1008987431361