Skip to main content
SpringerLink
Log in

Factors affecting pectate lyase activity during membrane filtration

  • Enzyme Recovery
  • Published:
Biotechnology Techniques

Summary

This paper presents problems encountered during ultrafiltration of pectate lyase (EC 4.2.2.2) of Erwinia chrysanthemi. Membrane adsorption was related to the chemical nature and charge of the membrane polymer. Simple pre-treatment such as coating with a lysine solution greatly improved recovery efficiency.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bertheau Y., Madgidi-Hervan E., Kotoujansky A., Nguyen-The C., Andro T. and Coleno A. (1984). Anal. Biochem. 139: 383–389.

    Google Scholar 

  • Blatt W.F., Feinberg M.P., Hopfenberg H.P. and Saravis C.A. (1965). Science 150: 224–226.

    Google Scholar 

  • Charm S.E. and Wong B.L. (1970). Science 170: 466–468.

    Google Scholar 

  • Charm S.E. and Wong B.L. (1981). Enzyme Microb. Technol. 3: 111–118.

    Google Scholar 

  • Cheryan M., Van Wyk P.J., Richarson T. and Olson N.F. (1976). Biotechnol. Bioeng. 18: 273–279.

    Google Scholar 

  • Clarke N.H. (1972). J. Dairy Sci. Tech. 7: 142–145.

    Google Scholar 

  • Deeslie W.D. and Cheryan M. (1982). Biotechnol. Bioeng. 24: 69–82.

    Google Scholar 

  • Dunlop C.E. (1985). Solids and Liquids handling - In: Comprehensive Biotechnology, M. Moo Young ed. Vol II, 237–271.

  • Georgiades J.A. (1981). Texas Reports Biol. Med. 41: 179–183.

    Google Scholar 

  • Kohlwey D.E. and Cheryan M. (1981). Enzyme Microb. Technol. 3: 64–68.

    Google Scholar 

  • Korus R.A. and Olson A.C. (1977). J. Food. Science 42: 258–260.

    Google Scholar 

  • Kotoujansky A. (1987). Ann. Rev. Phytopathol. 25: 405–430.

    Google Scholar 

  • Lee M.S. and Howell J.A. (1981). Inst. Chem. Eng. Ann. Research Meeting: 141–144.

  • Porter M.C. (1972). Biotechnol. Bioeng. Symosium n∘3: 115–144.

    Google Scholar 

  • Rombouts F.M. (1972). Doctoral Thesis; Wageningen.

  • Wang D.I.C., Sonoyama T. and Mateles R.I. (1968). Anal. Biochem. 18, 277.

    Google Scholar 

  • Wang D.I.C., Sinskey A.J. and Butterworth T.A. (1970). Enzyme Processing using ultrafiltation membranes. In: Membrane Science and Technology; Flinn J.E. eds. pp 98–119. Plenum Press.

Download references

Author information

Authors and Affiliations

  1. INRA, Lab. de recherches de technologie laitière, 65 rue de St Brieuc, 35042, Rennes cedex, France

    S. Denis, S. Terré, Y. Bertheau & P. Boyaval

  2. INA-PG, Laboratoire de pathologie végétale, 16 rue C. Bernard, 75231, 05, Paris cedex, France

    Y. Bertheau

Authors
  1. S. Denis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Terré
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. Bertheau
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Boyaval
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Denis, S., Terré, S., Bertheau, Y. et al. Factors affecting pectate lyase activity during membrane filtration. Biotechnol Tech 4, 127–132 (1990). https://doi.org/10.1007/BF00163286

Download citation

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00163286

Keywords

Search

Navigation