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A simple staining procedure for the characterisation of Basidiomycetes pellets by image analysis

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Biotechnology Techniques

Summary

A simple staining procedure, associated with image analysis, was used to discriminate two zones (the filamentous zones and the compacted cores) in aggregates of fungal biomass according to their accessibility for an agent of the medium. The application of the method to batch fermentations of Schizophyllum commune showed a higher proportion of loosely entangled particles (clumps) and thinner filamentous outer zones obtained at a high agitation speed (500 rpm) compared to low agitation speeds (120–200 rpm).

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References

  • Anke,T. (1989). In: Bioactive metabolites from microorganisms. Progress in industrial microbiology - vol.27, ed. M.E. Bushell and U. Gräfe, pp 51–66. Elsevier. London

    Google Scholar 

  • Clarke, D.S. (1962). Can. J. Microbiol. 8: 133–136

    Google Scholar 

  • Cox, P.W., Thomas, C.R. (1992). Biotechnol. Bioeng. 39: 945–952

    Google Scholar 

  • Fachet, J., Abel, G., Jusupova, S., Imre, S., Katona, E., Erdei, J., Szöllösi, J., Chihara, G. (1989). Int. J. Immunother. 4:167–176

    Google Scholar 

  • Furue, H. (1987). Drugs of today. 23(6): 335–346

    Google Scholar 

  • Hotta, H., Hagiwara, K., Tabata, K., Ito, W., Homma, M. (1993). Int.J.Immunopharmac. 15(1): 55–60

    Google Scholar 

  • Kiho, T., Shiose, Y., Nagai, K., Ukai, S. (1992a). Chem. Pharm. Bull. 40(8): 2110–2114

    Google Scholar 

  • Kiho,T., Katsuragawa, M., Nagai, K., Ukai, S. (1992b). Carbohydr. Res. 224: 237–243

    Google Scholar 

  • Pichon, D., Vivier, H., Pons, M.-N., (1993). In: Proceedings of the 5th International Conference on Computer Applications in Fermentation Technology. Keystone, Colorado, USA, 29 Mar–2 Apr 1992

  • Reichl, U., King, R., Gilles E.D. (1992). Biotechnol. Bioeng. 39: 164–170

    Google Scholar 

  • Song, C.H., Cho, K.Y., Nair, N.G. (1987). Mycologia 79(6): 866–876

    Google Scholar 

  • Thomas, C.R. (1992). Trends Biotechnol. 56(2): 204–207

    Google Scholar 

Download references

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Authors and Affiliations

  1. Bioprocédés, Ecole des Mines de St Etienne, 158 cours Fauriel, 2, St Etienne, France

    G. Durant & P. Formisyn

  2. Chimie Physique des Processus Industriels, Ecole des Mines de St Etienne, 158 cours Fauriel, 2, St Etienne, France

    G. Crawley

Authors
  1. G. Durant
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  2. G. Crawley
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  3. P. Formisyn
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Durant, G., Crawley, G. & Formisyn, P. A simple staining procedure for the characterisation of Basidiomycetes pellets by image analysis. Biotechnol Tech 8, 395–400 (1994). https://doi.org/10.1007/BF00154310

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  • DOI: https://doi.org/10.1007/BF00154310

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