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A novel device for the assessment of normal stresses time variations effects on suspended cell: Design and performances

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Biotechnology Techniques

Summary

A generator of strictly normal stress time variations was designed in order to study the membrane resistance to mechanical stress of various cell species. This system provides pressure drops within very short time periods: 22 MPa in 1.9 msec in liquid systems and 6 MPa in 10 msec in the presence of a gas. It also offers the possibility to study the effect of successive “compression / decompression” cycles on cell integrity and metabolism. A single decompression provoked no structural damage to the cells.

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Authors and Affiliations

  1. Département de Génie Biochimique et Alimentaire, Centre de Bioengéniérie Gilbert Durand, Lab. Ass. INRA, URA CNRS, 544, France

    C. Lafforgue-Delorme

  2. Institut National des Sciences Appliquées, Complexe Scientifique de Rangueil, 31077, Toulouse, France

    J. M. Bavouzet, C. Lafforgue-Delorme, C. Fonade & G. Goma

Authors
  1. J. M. Bavouzet
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  2. C. Lafforgue-Delorme
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  3. C. Fonade
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  4. G. Goma
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Bavouzet, J.M., Lafforgue-Delorme, C., Fonade, C. et al. A novel device for the assessment of normal stresses time variations effects on suspended cell: Design and performances. Biotechnol Tech 8, 663–670 (1994). https://doi.org/10.1007/BF00241695

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  • DOI: https://doi.org/10.1007/BF00241695

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