Summary
Molecular weight of PHB becomes low when using a hypochlorite extraction method for PHB separation. This disadvantage was overcome by adding sodium bisulfite which is an anti-oxidant. The molecular weight drop was decreased from 30 % to 14 % by adding sodium bisulfite. It was also found that raising the pH to 12 and the addition of a surfactant resulted in the improvement of the PHB purity.
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Roh, K.S., Yeom, S.H. & Yoo, Y.J. The effects of sodium bisulfite in extraction of PHB by hypochlorite. Biotechnol Tech 9, 709–712 (1995). https://doi.org/10.1007/BF00159234
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DOI: https://doi.org/10.1007/BF00159234