Skip to main content
SpringerLink
Log in

Isolation and characterization of X chromosome-derived DNA sequences from a dioecious plant Melandrium album

  • Published:
Chromosome Research Aims and scope Submit manuscript

Abstract

A number of X chromosome DNA sequences have been isolated from a dioecious plant, Melandrium album (syn. Silene latifolia),using chromosome microdissection followed by degenerate oligonucleotide-primed polymerase chain reaction (DOP–PCR) amplification. Six DNA clones were selected and further characterized by DNA/DNA hybridization techniques to check their copy numbers, sex-specific methylation patterns, species specificity and positions on chromosomes. These clones were moderately to highly repetitive (approximately 103–105 copies per haploid genome) and none of them gave a positive signal on Northern blots. One of the clones yielded a sex-specific methylation pattern: its abundant non-methylated CCGG island was found only in males. All the clones also hybridized to two closely related dioecious Melandrium species (M. rubrum and M. dicline). Nucleotide sequences of two X-derived clones showed a number of internal short direct repeats; one of them strikingly resembled a plant conservative telomere sequence (TTTAGGG).None of the clones hybridized to the X chromosome only, but all were localized at the telomeric heterochromatic regions (DAPI C-bands) of both arms of a vast majority of M. album chromosomes using the fluorescencein situ hybridization (FISH) technique. However, the non-homologousarm of the Y chromosome (contrary to the arm homologous to the X chromosome,possessing the pseudoautosomal region) showed neither a DAPI C-banding-stained heterochromatin nor a FISH signal with any of the DNA probes tested,thus indicating its evolutionary diversification.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Albini SM, Schwarzacher T (1992) In situlocalization of two repetitive DNA sequences to surface-spread pachytene chromosomes of rye. Genome 35: 551–559.

    Google Scholar 

  • Bedbrook J (1981) A plant nuclear DNA preparation procedure. Plant Mol Biol Newsl 2: 24.

    Google Scholar 

  • Bezdek M, Koukalová B, Kuhrová V, Vyskot B (1992) Differential sensitivity of CG and CCG DNA sequences to ethionine-induced hypomethylation of Nicotiana tabacum. FEBS Lett 300: 268–270.

    Article  PubMed  CAS  Google Scholar 

  • Charlesworth B (1991) The evolution of sex chromosomes. Science 251: 1030–1033.

    PubMed  CAS  Google Scholar 

  • Ciupercescu DD, Veuskens J, Mouras A, Ye D, Briquet M, Negrutiu I (1990) Karyotyping Melandrium album, a dioecious plant with heteromorphic sex chromosomes. Genome 33: 556–562.

    CAS  Google Scholar 

  • Copenhaver GP, Pikaard CS (1996) RFLP and physical mapping with an rDNA-specific endonuclease reveals that nucleolus organizer regions of Arabidopsis thalianaadjoin the telomeres on chromosome 2 and 4. Plant J 9: 259–272.

    Article  PubMed  CAS  Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Reporter 1: 19–21.

    CAS  Google Scholar 

  • Donnison IS, Siroky J, Vyskot B, Saedler H, Grant S (1996) Isolation of Y specific sequences from Silene latifoliaand mapping of male sex determining genes using representational difference analysis. Genetics 144: 1891–1899.

    Google Scholar 

  • Fajkus J, Reich J (1991) Evaluation of restriction endonuclease cleavage of plant nuclear DNA using contaminating chloroplast DNA. Folia Biol(Prague) 37: 224–226.

    Google Scholar 

  • Fajkus J, Královics R, Kovarík A, Fajkusová L (1995) The telomeric sequence is directly attached to the HRS 60 subtelomeric tandem repeat in tobacco chromosomes. FEBS Lett 364: 33–35.

    Article  PubMed  CAS  Google Scholar 

  • Grant S, Houben A, Vyskot B et al. (1994) Genetics of sex determination in flowering plants. Dev Genet 15: 214–230.

    Article  Google Scholar 

  • Irick H (1994) A new function for heterochromatin. Chromosoma 103: 1–3.

    Article  PubMed  CAS  Google Scholar 

  • Jeddeloh JA, Richards EJ (1996) mCCG methylation in angiosperms. Plant J 9: 579–586.

    Article  PubMed  CAS  Google Scholar 

  • Kamm A, Schmidt T, Heslop-Harrison JS (1994) Molecular and physical organization of highly repetitive, under-methylated DNA from Pennisetum glaucum. Mol Gen Genet 244: 420–425.

    Article  PubMed  CAS  Google Scholar 

  • Kenton A, Parokonny AS, Gleba YY, Bennett MD (1993) Characterization of the Nicotiana tabacumL. genome by molecular cytogenetics. Mol Gen Genet 240: 159–169.

    Article  PubMed  CAS  Google Scholar 

  • Kiss T, Kis M, Solomosy F (1989) Nucleotide sequence of a 25S rRNA gene from tomato. Nucleic Acids Res 17: 796.

    PubMed  CAS  Google Scholar 

  • Mastenbroek O, van Brederode J (1986) The possible evolution of Silene pratensisas deduced from present day variation patterns. Biochem Syst Ecol 14: 165–181.

    Article  CAS  Google Scholar 

  • Mulcahy DL, Weeden NF, Kesseli R, Caroll, SB (1992) DNA probes for the Y-chromosome of Silene latifolia, a dioecious angiosperm. Sex Plant Reprod 5: 86–88.

    Article  Google Scholar 

  • Pan WH, Houben A, Schlegel R (1993) Highly effective cell synchronization in plant roots by hydroxyurea and amiprophos-methyl or colchicine. Genome 36: 387–390.

    CAS  PubMed  Google Scholar 

  • Pich U, Fuchs J, Schubert I (1996) How do Alliaceaestabilize their chromosome ends in the absence of TTTAGGG sequences? Chrom Res 4: 207–213.

    Article  PubMed  CAS  Google Scholar 

  • Pich U, Houben A, Fuchs J, Meister A, Schubert I (1994) Utility of DNA amplified by degenerate oligonucleotide-primed PCR (DOP-PCR) from the total and defined chromosomal regions of the field bean. Mol Gen Genet 243: 173–177.

    PubMed  CAS  Google Scholar 

  • Rivin C (1986) Analyzing genome variation in plants. Methods Enzymol 118: 75–86.

    Article  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory Press.

    Google Scholar 

  • Široký J, Janousek B, Mouras A, Vyskot B (1994) Replication pattern of sex chromosomes in Melandrium albumfemale cells. Hereditas 120: 175–181.

    Article  Google Scholar 

  • Sugiura M, Shinozaki K, Zaita N, Kusuda M, Kumano M (1986) Clone bank of the tobacco (Nicotiana tabacum) chloroplast genome as a set of overlapping restriction endonuclease fragments: mapping of eleven ribosomal protein genes. Plant Sci 44: 211–216.

    Article  CAS  Google Scholar 

  • Telenius H, Carter NP, Bebb CE, Nordenskjüld M, Ponder BAJ, Tunnacliffe A (1992) Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer. Genomics 13: 718–725.

    Article  PubMed  CAS  Google Scholar 

  • Vagera J, Paulíková D, Doležel J (1994) The development of male and female regenerants by in vitroandrogenesis in dioecious plant Melandrium album. Ann Bot 73: 455–459.

    Article  Google Scholar 

  • Van Nigtevecht G, Prentice HC (1985) A note on the sex chromosomes of the Valencian endemic, Silene diclinis (Caryophyllaceae). Anales Jard Bot Madrid 41: 267–270.

    Google Scholar 

  • Vershinin AV, Schwarzacher T, Heslop-Harrison JS (1995) The large-scale genomic organization of repetitive DNA families at the telomeres of rye chromosomes. Plant Cell 7: 1823–1833.

    Article  PubMed  CAS  Google Scholar 

  • Veuskens J, Marie D, Brown SC, Jacobs M, Negrutiu I (1995) Flow sorting of the Y sex chromosome in the dioecious plant Melandrium album. Cytometry 21: 363–373.

    Article  PubMed  CAS  Google Scholar 

  • Vyskot B, Araya A, Veuskens J, Negrutiu I, Mouras A (1993) DNA methylation of sex chromosomes in a dioecious plant, Melandrium album. Mol Gen Genet 239: 219–224.

    PubMed  CAS  Google Scholar 

  • Westergaard M (1958) The mechanism of sex determination in dioecious flowering plants. Adv Genet 9: 217–281.

    PubMed  CAS  Google Scholar 

  • Winge Ö (1931) X-and Y-linked inheritance in Melandrium. Hereditas 15: 127–165.

    Article  Google Scholar 

  • Ye D, Installé P, Ciupercescu D et al. (1990) Sex determination in the dioecious Melandrium: I. First lessons from androgenic haploids. Sex Plant Reprod 3: 179–186.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biophysics, Czech Academy of Sciences, Kra´lovopolska´ 135, 612 65, Brno, Czech Republic

    Jiří Bůžek, Hana Koutníková, Karel Říha, Bohuslav Janousśek, Jiří Široký & Boris Vyskot

  2. Institute of Plant Genetics and crop Plant Research, Gatersleben, Germany

    Andreas Houben

  3. Max-Planck-Institute for Plant Breeding, Cologne, Germany

    Sarah Grant

Authors
  1. Jiří Bůžek
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hana Koutníková
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andreas Houben
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Karel Říha
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bohuslav Janousśek
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jiří Široký
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sarah Grant
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Boris Vyskot
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Boris Vyskot.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bůžek, J., Koutníková, H., Houben, A. et al. Isolation and characterization of X chromosome-derived DNA sequences from a dioecious plant Melandrium album. Chromosome Res 5, 57–65 (1997). https://doi.org/10.1023/A:1011693603279

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1011693603279

Search

Navigation