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Differentiation of Rhizoctonia AG-D Isolates from Turfgrass into Subgroups I and II Based on rDNA and RAPD Analyses

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Abstract

Binucleate Rhizoctonia anastomosis group (AG) D is the cause of rhizoctonia-patch and elephant-footprint diseases of zoysiagrass, and winter-patch disease of bentgrass. Rhizoctonia AG-D is also known as the causal pathogen of other diseases such as sharp-eye-spot of cereals, foot-rot of cereals and winter-stem-rot of mat rush. Isolates of AG-D have been divided into the two subgroups AG-D (I) and AG-D (II), based on the results of cultural characteristics and pathogenicity tests. Isolates obtained from zoysiagrass exhibiting symptoms of rhizoctonia-patch disease, from bentgrass with winter-patch disease, from wheat with foot-rot disease, and from mat rush with winter-stem-rot disease were reported to belong to subgroup AG-D (I). On the other hand, isolates obtained from zoysiagrass with elephant-footprint disease were assigned to subgroup AG-D (II). To confirm the existence of these two subgroups in AG-D, the genetic structure of AG-D isolates from turfgrass and other crops was compared. RFLP analysis of the ITS region from rDNA after digestion with the restriction enzymes EcoRI, HaeIII, HhaI, HinfI, and MboI separated AG-D isolates into two groups corresponding to AG-D (I) and AG-D (II). Furthermore, other AGs except AG-Q (AGs-A, Ba, Bb, C, E, F, G, I, K, L, O, P, and R. solani AG1-IC) did not have the same patterns that were seen for the two AG-D subgroups. AG-Q isolates from bentgrass showed the same patterns as AG-D (I). The results of the RAPD analysis also revealed the existence of two groups that corresponded to AG-D (I) and AG-D (II). These analyses revealed that Rhizoctonia AG-D isolates from turfgrass could be divided into two subgroups consistent with those based on cultural characteristics and pathogenicity. In addition, isolates of foot-rot disease of wheat and isolates of winter-stem-rot disease of mat rush whose cultural characteristics were the same as those of AG-D (I) also showed similar RFLP and RAPD patterns to those of AG-D (I) isolates from turfgrass.

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Authors and Affiliations

  1. Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu, 501-11, Japan

    Takeshi Toda, Mitsuro Hyakumachi & Koji Kageyama

  2. Laboratory of Plant Disease Science, Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu, 501-11, Japan (Phone

    Haruhisa Suga

  3. Institute of Genetic Ecology, Tohoku University, Sendai, 980-77, Japan

    Mitsuro Hyakumachi

  4. Institute for Green Science, 3335-2 Showa, Nagao, Okawa, Kagawa, 769-23, Japan

    Akemi Tanaka & Toshikazu Tani

Authors
  1. Takeshi Toda
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  2. Mitsuro Hyakumachi
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  3. Haruhisa Suga
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  4. Koji Kageyama
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  5. Akemi Tanaka
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  6. Toshikazu Tani
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Correspondence to Mitsuro Hyakumachi.

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Toda, T., Hyakumachi, M., Suga, H. et al. Differentiation of Rhizoctonia AG-D Isolates from Turfgrass into Subgroups I and II Based on rDNA and RAPD Analyses. European Journal of Plant Pathology 105, 835–846 (1999). https://doi.org/10.1023/A:1008736404263

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