Skip to main content
SpringerLink
Log in

Differential binding of a N-phenylformamidoxime compound in cell-free extracts of benzimidazole-resistant and-sensitive isolates of Venturia nashicola, Botrytis cinerea and Gibberella fujikuroi

  • Published:
Netherlands Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

Venturia nashicola isolates with a high level of resistance to carbendazim showed either increased sensitivity or were resistant to theN-phenylformamidoxime compoundN-(3,5-dichloro-4-propynyloyphenyl)-N′-methoxyformamidine (DCPF). Isolates with an intermediate or low level of carbendazim resistance were resistant to DCPF. Increased sensitivity to DCPF was also associated with a high level of carbendazim resistance inBotrytis cinerea but not with a moderate resistance level. Increased sensitivity to DCPF was not observed in carbendazim resistant isolates ofGibberella fujikuroi.

Binding of [14C]DCPF in cell-free mycelial extracts of the highly carbendazim-resistant and DCPF-sensitive isolate ofV. nashicola was higher than in those of DCPF-resistant isolates that were either highly-resistant, intermediately resistant, or weakly resistant to carbendazim or were sensitive to carbendazim. [14C]carbendazim binding in extracts of highly carbendazim-resistant isolates ofB. cinerea was lower than that in extracts of sensitive isolates, whereas [14C]DCFP binding was higher. A decreased [14C]carbendazim binding was also observed in extracts of carbendazim-resistant isolates ofG. fujikuroi, binding of [14C]DCPF, however, was similar in extracts of both carbendazim-resistant and sensitive isolates.

Samenvatting

Isolaten vanVenturia nashicola met een hoog niveau van resistentie tegen carbendazim vertoonden ofwel een verhoogde gevoeligheid voor deN-fenylformamidoxim verbinding dichloor-4-propynyloxyfenyl-N-methoxyformamidine (DCPF) of waren evenals matig en zwak carbendazim-resistente isolaten, resistent tegen deze verbinding. Ook carbendazim-resistente isolaten vanBotrytis cinerea bleken gevoelig te zijn geworden voor DCPF. BijGibberella fujikuroi was dit niet het geval.

Bindingsactiviteit voor [14C]DCPF in celvrije extracten van het hoog carbendazimresistente en DCPF-resistente isolaat vanV. nashicola bleek hoger te zijn dan bindingsactiviteit in extracten van de DCPF-resistente isolaten die ofwel hoog, matig of zwak-resistent of gevoelig waren voor carbendazim.

Bindingsactiviteit voor [14C]carbendazim in extracten van carbendazim-resistente isolaten vanB. cinerea was lager dan die in extracten van gevoelige isolaten en voor [14C]DCPF hoger. In extracten van carbendazim-resistente isolaten vanG. fujikuroi werd ook een lagere [14C]carbendazim bindingsactiviteit gevonden vergeleken met die van gevoelige isolaten; bindingsactiviteit voor [14C]DCPF bleek evenwel onveranderd.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Corbett, J.R., Wright, K. & Baillie, A.C., 1984. The Biochemical Mode of Action of Pesticides. Second Edition. Academic Press, London. 392 pp.

    Google Scholar 

  • Davidse, L.C. & Flach, W., 1977. Differential binding of methyl benzimidazol-2-yl carbamate to fungal tubulin as a mechanism of resistance to this antimitotic agent in mutant strains ofAspergillus nidulans. The Journal of Cell Biology 72: 174–193.

    PubMed  Google Scholar 

  • Davidse, L.C. & Flach, W., 1978. Interaction of thiabendazole with fungal tubulin. Biochimica et Biophysica Acta 543: 82–90

    PubMed  Google Scholar 

  • Elad, Y., Shabi, E. & Katan, T., 1988. Negative cross resistance between benzimidazole andN-phenylcarbamate fungicides and control ofBotrytis cinerea on grapes. Plant Pathology 37: 141–147.

    Google Scholar 

  • Gasztonyi, M., Josepovits, G., Molnár, A. & Hornok, L., 1987. Biochemical background of resistance to benomyl in genetically different strains ofFusarium oxysporum. Pesticide Biochemistry and Physiology 29: 17–24.

    Google Scholar 

  • Gessler, C., Sozzi, D. & Kern, H., 1980. Benzimidazol-fungizide: Wirkungsweise und Probleme. Berichte der Schweizerischen Botanischen Gesellschaft 90 (1/2): 45–54.

    Google Scholar 

  • Groves, J.D., Fox, R.T.V. & Baldwin, B.C., 1988a. Modes of action of carbendazim and ethylN-(3,5-dichlorophenyl)carbamate on field isolates ofBotrytis cinerea. Pages 397–402 in: Proceedings of the British Crop Protection Conference — Pests and Diseases, Vol. 1. BCPC Publications, Surrey, 1265 pp.

    Google Scholar 

  • Groves, J.D., Fox, R.T.V. & Baldwin, B.C., 1988b. Tubulin fromBotrytis cinerea, and the potential for development of an immunodiagnostic for benzimidazole resistance. Pages 415–420 in: Proceedings of the British Crop Protection Conference — Pests and Diseases, Vol. 1. BCPC Publications, Surrey, 1265 pp.

    Google Scholar 

  • Hartree, E.F., 1972. Determination of protein: a modification of the Lowry method that gives a linear photometric response. Analytical Biochemistry 48: 422–427.

    PubMed  Google Scholar 

  • Ishii, H. & Davidse, L.C., 1986. Decreased binding of carbendazim to cellular protein fromVenturia nashicola and its involvement in benzimidazole resistance. Pages 567–573 in: Proceedings of the British Crop Protection Conference — Pests and Diseases, Vol. 2. BCPC Publications, Surrey, 1255 pp.

    Google Scholar 

  • Ishii, H., Iwasaki, S., Sato, Z. & Inoue, I., 1989. Binding of cellular protein fromVenturia nashicola isolates to carbendazim and its relationship with the sensitivity toN-phenylcarbamate,N-phenylformamidoxime compounds, and rhizoxin. American Chemical Society Symposium Series: in press.

  • Ishii, H. & Takeda, H., 1988. Binding activity of cellular protein from benzimidazole resistant isolates of phytopathogenic fungi to aN-phenylformamidoxime compound. Page 310 in: Abstracts of the Fifth International Congress of Plant Pathology: 485 pp.

  • Ishii, H., Udagawa, H., Yanase, H. & Yamaguchi, A., 1985. Resistance ofVenturia nashicola to thiophanate-methyl and benomyl: build-up and decline of resistance in the field. Plant Pathology 34: 363–368.

    Google Scholar 

  • Ishii, H., & Van Raak, M., 1987. Genetic and biochemical aspects of increased sensitivity to formamidoxime compounds in benzimidazole resistant isolates ofVenturia nashicola. Page 158 in: Abstracts of the 11th International Congress of Plant Protection, 188 pp.

  • Ishii, H. & Van Raak, M., 1988. Inheritance of increased sensitivity toN-phenylcarbamates in benzimidazole-resistantVenturia nashicola. Phytopathology 78: 695–698.

    Google Scholar 

  • Ishii, H., Yanase, H. & Dekker, J., 1984. Resistance ofVenturia nashicola to benzimidazole fungicides. Mededelingen Faculteit der Landbouwwetenschappen, Rijksuniversiteit Gent, 49/2a: 163–172.

    Google Scholar 

  • Kato, T., Nakamura, S., Yamamoto, S., Takahashi, J., Noguchi, H. & Kamoshita K., 1985. Negatively correlated cross-resistance of benzimidazole-resistant isolates to S-165. (1) antifungal activity and control effect to benzimidazole-resistant isolates (In Japanese). Page 108 in: Abstracts of the 10th Annual Meeting of Pesticide Science Society of Japan, 162 pp.

  • Kato, T., Suzuki, K., Takahashi, J. & Kamoshita, K., 1985. Negatively correlated cross-resistance between benzimidazole fungicides and methylN-(3,5-dichlorophenyl)carbamate. Journal of Pesticide Science 9: 489–495.

    Google Scholar 

  • Leroux, P., Gredt, M., Massenot, F. & Kato, F., 1985. Activité du phenylcarbamate: S 32165 surBotrytis cinerea, agent de la pourriture grise de la vigne. Pages 443–446 in: M. Smith (Ed.), Proceedings of the Bordeaux Mixture Centenary Meeting. Vol. 2. BCPC Publications, Croydon, 504 pp.

    Google Scholar 

  • Nakamura, S., Kato, T., Noguchi, H., Takahashi, J. & Kamoshita, K., 1986. Biological activity and mode of action of a resistance breaker, diethofencarb. 3E-01 in: Abstracts of the Sixth International Congress of Pesticide Chemistry.

  • Nakata, A., Sano, S., Hashimoto, S., Hayakawa, K., Nishikawa, H. & Yasuda, Y., 1987. Negatively correlated cross-resistance toN-phenylformamidoximes in benzimidazole-resistant phytopathogenic fungi. Annals of the Phytopathological Society of Japan 53: 659–662.

    Google Scholar 

  • Tripathi, R.K. & Schlösser, E., 1982. The mechanism of resistance ofBotrytis cinerea to methyl benzimidazol-2-yl carbamate (MBC). Journal of Plant Diseases and Protection 89: 151–156.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Fruit Tree Research Station, 305, Tsukuba, Ibaraki, Japan

    H. Ishii & H. Takeda

Authors
  1. H. Ishii
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Takeda
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Contribution No.: A-242 Fruit Tree Res. Stn.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ishii, H., Takeda, H. Differential binding of a N-phenylformamidoxime compound in cell-free extracts of benzimidazole-resistant and-sensitive isolates of Venturia nashicola, Botrytis cinerea and Gibberella fujikuroi. Netherlands Journal of Plant Pathology 95 (Suppl 1), 99–108 (1989). https://doi.org/10.1007/BF01974289

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01974289

Additional keywords

Search

Navigation