Abstract
Rooted cuttings of white flowered cv. `Purnima' and red flowered cv. `Colchi Bahar' of Chrysanthemum morifolium were treated with gamma rays. Sectorial somatic mutations in flower colour were detected in both the cvs. Mutated ray florets (yellow colour for both the cvs) were cultured on agar-solidified Murashige & Skoog basal medium supplemented with sucrose and different combinations of BAP/kinetin and NAA. Direct shoot organogenesis was noticed within 2 weeks of culture initiation. SEM studies confirmed this direct shoot differentiation. The best regeneration was obtained on medium supplemented with 0.2 mg/l NAA and 0.5 mg/lBAP. Shoots regenerated from mutated ray florets were rooted in vitro and transferred to the field. These plants flowered true-to-explant floret colour and shape. These isolated yellow mutants were maintained vegetatively have proved to be true-to-type in two successive generations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bush, S.R., E.D. Earle & R.W. Langhans, 1976. Plantlets from petal segments, petal epidermis and shoot tips of the periclinal chimera, Chrysanthemum morifolium 'Indianpolis'. Amer J Bot 63: 729-737.
Datta, S.K., 1988. Chrysanthemum cultivar evolved by induced mutation at National Botanical Research Institute, Lucknow. The Chrysanthemum 44: 72-75.
Datta, S.K. & B.K. Banerji, 1990. 'Los Banos Variegata'-A new double bracted chlorophyll variegated bougainvillea induced by gamma rays. J Nuclear Agric Biol 19: 134-136.
Datta, S.K., 1997. Ornamental plants: Role of mutation. Daya Publishing House, New Delhi, India.
De Jong & J.B.M. Custers, 1986. Induced changes in growth and flowering of chrysanthemum after irradiation and in vitro culture of pedicels and petal epidermis. Euphytica 35: 137-148.
Malaure, R.S., G. Barclay, J.B. Power & M.R. Davey, 1991. The production of novel plants from florets of Chrysanthemum morifolium using tissue culture 1. Shoot regeneration from ray florets and somaclonal variation exhibited by the regenerated plants. J Plant Physiol 139: 8-13.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bio-assays with tobacco tissue culture. Physiol Plant 15: 473-491.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mandal, A., Chakrabarty, D. & Datta, S. In vitro isolation of solid novel flower colour mutants from induced chimeric ray florets of chrysanthemum. Euphytica 114, 9–12 (2000). https://doi.org/10.1023/A:1003960906646
Issue Date:
DOI: https://doi.org/10.1023/A:1003960906646