Skip to main content
SpringerLink
Log in

Hybridization of radish (Raphanus sativus L.) and oilseed rape (Brassica napus L.) through a flower-culture method

  • Published:
Euphytica Aims and scope Submit manuscript

Summary

Hybridization between radish and oilseed rape has been cumbersome, requiring elaborate embryo rescue techniques. With a modified flower culture method, we have achieved successful hybridization between radish and (transgenic) oilseed rape without the laborious and technically demandingin vitro ovule or embryo rescue techniques.

The hybrid nature of the intergeneric hybrids was demonstrated using morphological traits, and DNA analyses. The described method will facilitate the generation ofRaphanobrassica hybrids useful for biosafety studies of the potential for transgenes to spread in weedyCruciferae as well as for breeding programs aimed at introducing useful radish genes, e.g. nematode resistance genes, into oilseed rape.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aarts, M.G.M., W.G. Dirkse, W.J. Stiekema & A. Pereira, 1993. Transposon tagging of a male sterility gene inArabidopsis. Nature 363: 715–717.

    Google Scholar 

  • Arumuganathan, K. & E.D. Earle, 1991. Estimation of nuclear DNA content of plants by flow cytometry. Plant Molec. Biol. Reporter 9 (3): 229–241.

    Google Scholar 

  • Becker, Th., 1951. Siebenjährige blütenbiologische Studien an den CruciferenBrassica napus L.,Brassica rapa L.,Brassica oleracea L.,Raphanus L. undSinapis L. II Teil. Z. Pflanzenzüchtung 31: 72–103.

    Google Scholar 

  • Bino, R.J., S. Lanteri, H.A. Verhoeven & H.L. Kraak, 1993. Flow cytometric determination of nuclear replication stages in seed tissues. Annals of Botany 72: 181–187.

    Google Scholar 

  • Block, M. de, J. Botterman, M. Vandewiele, J. Dockx, C. Thoen, V. Gosselé, N. Rao Movva, C. Thompson, M. van Montagu & J. Leemans, 1987. Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO J. 6 (9): 2513–2518.

    Google Scholar 

  • Chèvre, A.M., F. Eber, H. Brun, J. Plessis, C. Primard & M. Renard, 1991. Cytogenetic studies ofBrassica napus-Sinapis alba hybrids from ovary culture and protoplast fusion. Attempts to introduceAlternaria resistance into rapessed. Proc. GCIRC Eighth International Rapessed Congress July 9 to 11 1991, Saskatoon, Canada pp. 346–351.

  • Chopinet, R., 1944. Hybrides intergénériquesRaphano-Brassica. Revue Horticole. Paris, 116: 98–100.

    Google Scholar 

  • Clauss, E., 1978. Allohexaploide Gattungsbastarde vom TypBrassico-Raphanobrassica. Archiv für Züchtungsforschung Berlin 8 (5): 297–302.

    Google Scholar 

  • Dellaporta, S.L., J. Wood & J.B. Hicks, 1983. A plant DNA minipreparation: version II. Plant Molec. Biol. Reporter 1 (4): 19–21.

    Google Scholar 

  • Demeke, T., R.P. Adams & R. Chibbar, 1992. Potential taxonomic use of random amplified polymorphic DNA (RAPD): a case study inBrassica. Theor. Appl. Genet. 84: 990–994.

    Google Scholar 

  • Dolstra, O., 1982. Synthesis and fertility ofBrassicoraphanus and ways of transferringRaphanus characters toBrassica. PhD thesis Agricultural University Wageningen, The Netherlands. Agric. Res. Rep. 917, Pudoc, Wageningen, 90 pp.

    Google Scholar 

  • Hagimori, M., M. Nagaoka, N. Kato & H. Yoshikawa, 1992. Production and characterization of somatic hybrids between the japanese radish and cauliflower. Theor. Appl. Genet. 84: 819–824.

    Google Scholar 

  • Kato, M. & S. Tokumasu, 1976. The mechanism of increased seed fertility accompanied with the change of flower colour inBrassicoraphanus. Euphytica 25: 761–767.

    Google Scholar 

  • Kramer, C., J. DiMaio, G.K. Carswell & R.D. Shillito (1993). Selection of transformed protoplast derived Zea mays colonies with phosphinothricin and a novel assay using the pH indicator chlorophenol red. Planta 190: 454–458.

    Google Scholar 

  • Lardon, A., A.M. Triboi-Blondel & C. Dumas. 1993. A model for studying pollination and pod development inBrassica napus: the culture of isolated flowers. Sex. Plant Reprod 6: 52–56.

    Google Scholar 

  • Lelivelt, C.L.C. & F.A. Krens, 1992. Transfer of resistance to the beet cyst nematode (Heterodera schachtii Schm) into theBrassica napus gene pool through intergeneric somatic hybridization withRaphanus sativus L. Theor. Appl. Genet. 83: 887–894.

    Google Scholar 

  • Lelivelt, C.L.C., W. Lange & O. Dolstra, 1993a. Intergeneric crosses for the transfer of resistance to the beet cyst nematode fromRaphanus sativus toBrassica napus. Euphytica 68 (1–2): 111–120.

    Google Scholar 

  • Lelivelt, C.L.C., E.H.M. Leunissen, H. J. Frederiks, J.P.F.G. Helsper & F.A. Krens, 1993b. Transfer of resistance to the beet cyst nematode (Heterodera schachtii Schm.) fromSinapis alba L. (white mustard) to theBrassica napus L. gene pool by means of sexual and somatic hybridization. Theor. Appl. Genet. 85 (5–6): 688–696.

    Google Scholar 

  • Lelivelt, C.L.C., 1993. Introduction of beet cyst nematode resistance fromSinapis alba L. andRaphanus sativus L. intoBrassica napus L. (oil-seed rape) through sexual and somatic hybridization. PhD Thesis Agricultural University Wageningen. The Netherlands, pp. 141.

    Google Scholar 

  • McNaughton, I.H., 1973. Synthesis and sterility ofRaphanobrassica. Euphytica 22: 70–88.

    Google Scholar 

  • McNaughton, I.H. & C.L. Ross, 1978. Inter-specific and intergeneric hybridization in theBrassicae with special emphasis on the improvement of forage crops. Ann. Rep. Scottish Plant Breeding Station, pp. 75–110.

  • Mizushima, U., 1980. Genome analysis inBrassica and allied genera. In: S. Tsunoda, K. Hinata & C. Gomez-Campos (Eds),Brassica crops and wild allies-Biology and breeding, Japan Scientific Societies Press, Tokyo, pp. 89–106.

    Google Scholar 

  • Murakami, T., H. Anzai, S. Imai, A. Satoh, K. Nagaoka & C.J. Thompson, 1986. The bialaphos biosynthetic genes ofStreptomyces hygroscopicus: molecular cloning and characterization of the gene cluster. Molecular and General Genetics 205: 42–50.

    Google Scholar 

  • Marashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.

    Google Scholar 

  • Namai, H., 1978. Aspect on transfer of economic characters by means of interspecific and intergeneric crossing in cruciferous crops. Proceedings 5th International Rapessed Conference, Malmö, Sweden, Vol 1, June 12–16: 127–130.

    Google Scholar 

  • Namai, H., 1976. Cytogenetic and breeding studies on transfer of economic characters by means of interspecific and intergeneric crossing in the tribeBrassiceae ofCruciferae. Memoires of the Fac. Agric., Tokyo Univ. of Education 22: 101–171.

    Google Scholar 

  • Paulmann, W. & G. Röbbelen, 1988. Effective transfer of cytoplasmic male sterility from radish (Raphanus sativus L.) to rape (Brassica napus L.). Plant Breeding 100: 299–309.

    Google Scholar 

  • Prakash, S. & K. Hinata, 1980. Taxonomy, cytogenetics and origin of cropBrassicas, a review. Opera Botanica 55: 1–57.

    Google Scholar 

  • Rosén, B. & M. Olin-Fatih, 1993.Raphanobrassica somatic hybrids betweenBrassica napus L. andRaphanus sativus L. with aneuploid, chimaeric chromosome numbers. Sveriges Utsädesförenings Tidskrift 103: 147–153.

    Google Scholar 

  • Scheffler, J.A. & P.J. Dale, 1994. Opportunities for gene transfer from transgenic oilseed rape (Brassica napus) to related species. Transgenic Research 3: 263–278.

    Google Scholar 

  • Tachibana, K., T. Watanabe, Y. Sekizuwa & T. Takematsu, 1986. Accumulation of ammonia in plants treated with bialaphos. J. Pesticide Sci. 11: 33–37.

    Google Scholar 

  • Takeshita, M., M. Kato & S. Tokumasu, 1980. Application of ovule culture to the production of intergeneric or interspecific hybrids inBrassica andRaphanus. Jap. J. Genet. 55: 373–387.

    Google Scholar 

  • Thierfelder, A., E. Hackenberg & W. Friedt, 1992. Transfer of nematode resistance fromRaphanus to rapessed via interspecific hybridization and embryo rescue. Proc. 13th Eucarpia congress Angers, France, pp. 327–328.

  • Thompson, C., N. Movva, R. Tizard, R. Crameri, J. Davies, M. Lauwereys & J. Botterman, 1987. Characterization of the herbicide resistance gene ‘bar’ fromStreptomyces hygroscopicus. EMBO J. 6 (9): 2519–2523.

    Google Scholar 

  • Thormann, C.E., M.E. Ferreira, L.E.A. Camargo, J.G. Tivang & T.C. Osborn, 1994. Comparison of RFLP and RAPD markers to estimating genetic relationships within and among cruciferous species. Theor. Appl. Genet. 88: 973–980.

    Google Scholar 

  • Tokumasu, S., 1965. On the origin of the matromorphic plants ofBrassica japonica obtained from the cross betweenBrassica andRaphanus. J. Jap. Soc. Hort. Sci. 34: 223–231.

    Google Scholar 

  • Turesson, G. & H. Nordenskiöld, 1943. Chromosome doubling and cross combinations in some cruciferous plants. Annals of the Agric. College of Sweden 11: 201–206.

    Google Scholar 

  • U, N., 1935. Genome analysis inBrassica with special reference to the experimental formation ofB. napus and peculiar mode of fertilization. Jap. J. Bot. 7: 389–452.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, The Netherlands

    Peter L. J. Metz, Jan-Peter Nap & Willem J. Stiekema

Authors
  1. Peter L. J. Metz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jan-Peter Nap
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Willem J. Stiekema
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Metz, P.L.J., Nap, JP. & Stiekema, W.J. Hybridization of radish (Raphanus sativus L.) and oilseed rape (Brassica napus L.) through a flower-culture method. Euphytica 83, 159–168 (1995). https://doi.org/10.1007/BF01678044

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01678044

Key words

Search

Navigation