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Expression of foreign genes in sunflower (Helianthus annuus L.) — evaluation of three gene transfer methods

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Summary

Suitable sunflower tissues and cells were transformed either by direct gene transfer into protoplasts, particle bombardment, or Agrobacterium co-culture. While all techniques allowed efficient short-term or transient expression of the introduced gene(s) in the respective tissues, stable transformation was only observed after transformation with Agrobacterium. The latter technique was suitable for the production of transgenic callus from seedling cotyledons and occasional shoots with chimaeric expression of the transgene. Detailed analysis of the interaction of Agrobacterium with this explant showed that infection efficiency was critically dependent on the co-culture conditions, and that the preferentially-transformed cells were not the ones competent for regeneration.

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Abbreviations

BAP:

benzyl adenine

CAT:

chloramphenicol acetyl transferase

2,4-D:

2,4-dichloro phenoxy acetic acid

GUS-β:

D-glucuronidase

MS:

medium according to Murashige & Skoog (1962)

NAA:

naphthalene acetic acid

NPTII:

neomycin phospho transferase II

PEG:

polyethylene glycol

PIG:

particle inflow gun

SH:

medium according to Schenk & Hildebrandt (1972)

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Author notes

  1. Monique Burrus

    Present address: Laboratoire de Biologie et Physiologie Végétales, UFR Sciences, Université de Reims, BP 374, 51062, Reims, France

  2. Brigitte Damm

    Present address: MOGEN Intl., Einsteinweg 97, 2333 LB, Leiden, The Netherlands

  3. Ana Maria Bravo-Angel

    Present address: Friedrich-Miescher-Institut, Postfach 273, 4002, Basel, Switzerland

Authors and Affiliations

  1. Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique et Université Louis Pasteur, 12, Rue de Général Zimmer, 67084, Strasbourg Cedex, France

    Hélène Laparra, Monique Burrus, Reiner Hunold, Brigitte Damm, Ana Maria Bravo-Angel, Roberte Bronner & Günther Hahne

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  1. Hélène Laparra
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  2. Monique Burrus
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  3. Reiner Hunold
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  4. Brigitte Damm
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  6. Roberte Bronner
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  7. Günther Hahne
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Laparra, H., Burrus, M., Hunold, R. et al. Expression of foreign genes in sunflower (Helianthus annuus L.) — evaluation of three gene transfer methods. Euphytica 85, 63–74 (1995). https://doi.org/10.1007/BF00023931

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