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Some methodological aspects of apple transformation by Agrobacterium

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Summary

Leaf explants of apple cvs Gala and Golden Delicious were infected with the Agrobacterium tumefaciens strain AGL0(pMOG410). The effects of a 2 d preculture of the explants before infection and the addition of 5-azacytidine to the selection medium were studied. The percentages of GUS-positive explants after 5 w did not significantly alter due to these treatments. One of the ‘Gala’ shoots, which was removed from a leaf explant cultured for 8 w on selection medium, proved to be GUS-positive and will be analyzed further. In general, however, it should be concluded that regeneration of transgenic shoots directly from leaf tissue was not very effective.

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Abbreviations

MS:

Murashige and Skoog medium

GD:

‘Golden Delicious’

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Authors and Affiliations

  1. Department of Cell Biology, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, NL-6700 AA, Wageningen, The Netherlands

    J. G. Schaart & K. J. Puite

  2. Fruitgrowing Research Institute, Plovdiv, Bulgaria

    L. Kolova

  3. Institute of Cell Biology and Genetic Engineering, Kiev, The Ukraine

    N. Pogrebnyak

Authors
  1. J. G. Schaart
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  2. K. J. Puite
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  3. L. Kolova
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  4. N. Pogrebnyak
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Schaart, J.G., Puite, K.J., Kolova, L. et al. Some methodological aspects of apple transformation by Agrobacterium . Euphytica 85, 131–134 (1995). https://doi.org/10.1007/BF00023941

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