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Biochemical differences in lateral muscle of wild and farmed gilthead sea bream (series Sparus aurata L.)

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Abstract

Red and white muscle from specimens of wild and farmed gilthead sea bream (Sparus aurata) were analyzed for histochemical ATPase activity, total protein content, fatty acids, trace element concentrations and myosin isoforms. The fibre type composition of muscle samples was confirmed histochemically by the ATPase reaction, which did not show any differences between the two groups of animals. Myosin ATPase activities, myosin and protein yields were significantly higher in white muscle than in the red muscle and for the red muscle the latter two parameters were higher in wild fish. Fatty acid profiles revealed differences between the two groups of animals, probably because of the fatty acid composition of the diets. Zinc, copper and iron concentrations were higher in red muscle than in white muscle; muscles from wild fish were significantly richer in trace elements. No separation of fast and slow heavy chains of myosin could be obtained on SDS-gel electrophoresis, but two dimensional electrophoresis revealed the presence of three light chains in white muscle (LC1F, LC2F, LC3F), and two main types in red muscle (LC1S, LC2S). Small, variable percentages of LC3F were found in the red muscle samples, especially in the wild fish. It is concluded that the different environmental conditions, experienced by wild and farmed fish, have significantly influenced the biochemical composition of their lateral muscle.

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Authors and Affiliations

  1. Department of Biochemistry, Veterinary Biochemistry Section, University of Bologna, Ozzano Emilia, Bologna, Italy (Phone

    E. Carpene

  2. Department of Experimental Biomedical Sciences, CNR Unit for Muscle Biology and Physiopathology, Via G. Colombo 3 (ex Via Trieste 75), 35100, Padova, Italy

    B. Martin & L. Dalla Libera

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  1. E. Carpene
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Carpene, E., Martin, B. & Dalla Libera, L. Biochemical differences in lateral muscle of wild and farmed gilthead sea bream (series Sparus aurata L.). Fish Physiology and Biochemistry 19, 229–238 (1998). https://doi.org/10.1023/A:1007742328964

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