Skip to main content
Log in

Studies on teleost corpuscles of Stannius: Physiological and biochemical aspects of synthesis and release of hypocalcin in trout, goldfish and eel

  • Published:
Fish Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

Hypercalcemia (induced by CaCl2-injection or seawater exposure of the fish) reduced the hypocalcin content of corpuscles of Stannius (CS) in trout, goldfish and eel; concomitantly the synthetic activity of CS of hypercalcemic fish, as determinedin vitro, was enhanced. The monomeric forms of prohypocalcin and of hypocalcin of trout and goldfish are 32 and 28 kDA Mr glycoprotein species respectively; those of the eel are 2 kDa bigger,viz. 34 and 30 kDa respectively. Moreover, eel CS producein vitro an enigmatic 70 kDa glycoprotein with affinity for concanavalin-A. It is concluded that plasma calcium levels control storage and synthesis rates of hypocalcin in the CS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References cited

  • Aida, K., Nishioka, R.S. and Bern, H.A. 1980. Degranulation of the Stannius corpuscles of coho salmon (Oncorhynchus kisutch) in response to ionic changesin vitro. Gen. Comp. Endocrinol. 41: 305–313.

    Article  PubMed  CAS  Google Scholar 

  • Anderson, D.J. and Blobel, G. 1983. Immunoprecipitation of proteins from cell-free translations. Meth. Enzymol. 96: 111–121.

    Article  PubMed  CAS  Google Scholar 

  • Bonner, W.J. and Laskey, R.A. 1974. A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 46: 83–88.

    Article  PubMed  CAS  Google Scholar 

  • Butkus, A., Roche, P.J., Fernley, R.T., Haralambidis, J., Penschow, J.D., Ryan, G.B., Trahair, J.F., Tregear, G.W. and Coghlan, J.P. 1987. Purification and cloning of a corpuscles of Stannius protein fromAnguilla australis. Mol. Cell. Endocrinol. 54: 123–133.

    Article  PubMed  CAS  Google Scholar 

  • Fenwick, J.C. 1982. Some evidence concerning the nature of the hypocalcemic factor in the corpuscle of Stannius.In Comparative Endocrinology of Calcium Regulation. pp. 167–172. Edited by C. Oguro and P.K.T. Pang. Japan Scientific Press, Tokyo.

    Google Scholar 

  • Kaneko, T., Fraser, R.A., Labedz, T., Harvey, S. Lafeber, F.P.J.G. and Pang, P.K.T. 1988. Characterization of antisera raised against hypocalcin (teleoscalcin) purified from corpuscles of Stannius of rainbow trout,Salmo gairdneri. Gen. Comp. Endocrinol. 69: 238–245.

    Article  PubMed  CAS  Google Scholar 

  • Laemmli, U.K. 1970. Cleavage of the structural proteins during assembly of the head of bacteriophage T4. Nature, Lond. 227: 680–685.

    Article  CAS  Google Scholar 

  • Lafeber, F.P.J.G., Hanssen, R.G.J.M., Choy, Y.M., Flik, G., Hermann-Erlee, M.P.M., Pang, P.K.T. and Wendelaar Bonga, S.E. 1988. Identification of hypocalcin (teleocalcin) isolated from trout Stannius corpuscles. Gen. Comp. Endocrinol. 69: 19–30.

    Article  PubMed  CAS  Google Scholar 

  • Lafeber, F.P.J.G. and Perry, S.F. 1988. Experimental hypercalcemia induces hypocalcin release and inhibits branchial Ca2+ influx in freshwater trout. Gen. Comp. Endocrinol. 72: 136–143.

    Article  PubMed  CAS  Google Scholar 

  • Lopez, E., Tisserand-Jochem, E.-M., Eyquem, A., Milet, C., Hillyard, C., Lallier, F., Vidal, B. and MacIntyre, I. 1984. Immunocytochemical detection in eel corpuscles of Stannius of a mammalian parathyroid-like hormone. Gen. Comp. Endocrinol. 53: 28–36.

    Article  PubMed  CAS  Google Scholar 

  • Majzoub, J.A., Dee, P.C. and Habener, J.F. 1982. Cellular and cell-free processing of parathyroid secretory proteins. J. Biol. Chem. 257: 3581–3588.

    PubMed  CAS  Google Scholar 

  • Pang, P.K.T., Pang, R.K. and Sawyer, H. 1974. Environmental calcium and the sensitivity of killifish (Fundulus heteroclitus) in bioassays for the hypocalcemic response to Stannius corpuscles from killifish and cod (Gadus morhua). Endocrinology 94: 548–555.

    Article  PubMed  CAS  Google Scholar 

  • Roelfzema, H. and Van Erp, P.E.J. 1983. Glycoprotein composition of psoriatic epidermis in relation to growth control. J. Invest. Dermatol. 80: 20–28.

    Article  PubMed  CAS  Google Scholar 

  • Tisserand-Jochem, E.M., Lopez, E., Milet, C., Vidal, B., Magnac, C., Eyquem, A. and Cohn, D.V. 1987. Co-localization and secretion of parathyrin of Stannius corpuscles (immunoreactive parathyroid hormone) and of secretory glycoproteins including secretory protein-I in the European eel (Anguilla anguilla L.). Bone Min. 2: 163–174.

    CAS  Google Scholar 

  • Wagner, G.F., Hampong, M., Park, C.M. and Copp, D.H. 1986. Purification, characterization, and bioassay of teleocalcin, a glycoprotein from salmon corpuscles of Stannius. Gen. Comp. Endocrinol. 63: 481–491.

    Article  PubMed  CAS  Google Scholar 

  • Wendelaar Bonga S.E. and Pang, P.K.T. 1986. Stannius corpuscles.In Vertebrate Endocrinology, Fundamentals and Biomedical Implications. pp. 439–464. Edited by P.K.T. Pang and M.P. Schreibman. Academic Press, New York.

    Google Scholar 

  • Wendelaar Bonga, S.E., Smits, P.W.J.M., Flik, G., Kaneko, T. and Pang, P.K.T. 1989. Immunocytochemical localization of hypocalcin in the endocrine cells of the corpuscles of Stannius in three teleost species. Cell Tiss. Res. (In press)

  • Wohlfarter, T., Fisher-Colbrie, R., Hogue-Angeletti, R., Eiden, L.E. and Winkler, H. 1988. Processing of chromagranin A within chromaffin granules starts at C- and N-terminal cleavage sites. FEBS Lett. 231: 67–70.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Flik, G., Labedz, T., Lafeber, F.P.J.G. et al. Studies on teleost corpuscles of Stannius: Physiological and biochemical aspects of synthesis and release of hypocalcin in trout, goldfish and eel. Fish Physiol Biochem 7, 343–349 (1989). https://doi.org/10.1007/BF00004727

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00004727

Keywords

Navigation