Abstract
Molecular diversity in Saccharum complex was studied using 195 RAPD markers generated by 12 random primers. Among the Saccharum species, S. officinarum showed a low level of genetic diversity while S. sinense was found to be more diverse. Six taxonomical groups were clearly resolved in the cluster analysis. S. officinarum, S. robustum, S. spontaneum and Erianthus spp. formed discrete groups. S. barberi and S. sinense formed a single cluster, so also Narenga and Sclerostachya. S. officinarum was found to be closer to S. robustum and distant from S. spontaneum. Among the related genera, Sclerostachya was closer to Saccharum while Erianthus was found to be highly divergent from all the Saccharum species. Six of the primers used generated RAPD fragments unique to Erianthus. It is suggested that the Erianthus spp. can contribute substantially towards sugarcane varietal improvement in view of its greater divergence with Saccharum.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Al-Janabi, S.M., M. McClelland, C. Petersen & B.W.S. Sobral, 1994. Phylogenetic analysis of organellar DNA sequences in the Andropogoneae: Saccharinae. Theor. Appl. Genet. 88: 933–944.
Arceneaux, G., 1965. Cultivated sugarcanes of the world and their botanical derivation. Proc. Int. Soc. Sugar Cane Technol. (Puerto Rico) 12: 844–854.
Artschwager, E., 1954. A taxonomic study of Saccharum sinense Roxb. and S. barberi Jeswiet. USDA Tech. Bull. No. 1089, p. 87.
Barber, C.A., 1916. The classification of indigenous Indian canes. Agric. J. India, II (4): 371–376.
Brandes, E.W., 1958. Origin, classification and characteristics. In: Artschwager, E. & E.W. Brandes (Eds.), Sugarcane (Saccharum officinarum L). Agric. Handbk. (U.S. Dept. Agric.) 122, pp. 1–35.
Burnquist, W.L., M.E. Sorrells & S.D. Tanksley, 1995. Characterization of genetic variability in Saccharum germplasm by means of Restriction Fragment Length Polymorphism (RFLP) analysis. Proc. Int. Soc. Sugarcane Technol. 21: 355–365.
Daniels, J., P. Smith, N. Paton & C.A. Williams, 1975. The origin of the genus Saccharum. Sugarcane Breed. Newsl. 36: 24–39.
Daniels, J., J.B. Ashley & C. Daniels, 1980. The 1914 sugarcane collecting expedition to New Guinea. Sugarcane Breed. Newsl. 43: 24–38.
Daniels, J. & B.T. Roach, 1987. Taxonomy and evolution. In: Heinz, D.J. (Ed.), Sugarcane improvement through breeding, pp. 7–84. Elsevier Press, Amsterdam.
D'Hont, A., Y.H. Lu, P. Feldmann & J.C. Glaszmann, 1993. Cytoplasmic diversity in sugarcane revealed by heterologous probes. Sugar Cane 1: 12–15.
Glaszmann, J.C., J.L. Noyer, A. Fautret, P. Feldmann & C. Lanaud, 1989. Biochemical genetic markers in sugarcane. Theor. Appl. Genet. 78: 537–543.
Grassl, C.O., 1972. Taxonomy of Saccharum relatives: Sclerostachya, Narenga and Erianthus. Proc. Int. Soc. Sugar Cane Technol. 14: 240–248.
Grassl, C.O., 1977. The origin of the sugar producing cultivars of Saccharum. Sugarcane Breed. Newsl. 39: 8–33.
Janaki-Ammal, E.K., 1941. Intergeneric hybrids of Saccharum. J Genet 41: 217–253.
Li, H.W., C.S. Loh & C.L. Lee, 1948. Cytological studies of sugarcane and its relatives. 1. Hybrids between Saccharum officinarum, Miscanthus japonicus and Saccharum spontaneum. Bot. Bull. Acad. Sin. (Taipei) 2: 147–160.
Lu Y.H., A. D'Hont, D.I.T. Walker, P.S. Rao, P. Feldmann & J. C. Glaszmann, 1994. Relationships among ancestral species of sugarcane revealed with RFLP using single-copy maize nuclear probes. Euphytica 78: 7–18.
Moore, P.H. & J.E. Irvine, 1991. Genomic mapping of sugarcane and its potential contribution to improvement and to selection of new varieties. Proc. South African Sugar Technol. Assoc., June 1991, pp. 96–102.
Mukherjee, S.K., 1957. Origin and distribution of Saccharum. Bot. Gaz. 119: 55–61.
Nair, S., J.S. Bentur, U. Prasad Rao & M. Mohan, 1995. DNA markers tightly linked to a gall midge resistance gene (Gm2) are potentially useful for marker-aided selection in rice breeding. Theor. Appl. Genet. 91: 68–73.
Panje, R.R., 1954. Studies in Saccharum spontaneum and allied grasses. III. Recent exploration for Saccharum spontaneum and related grasses in India. Proc. Int. Soc. Sugarcane Technol. 8: 491–504.
Parthasarathy, N., 1948. Origin of noble sugarcanes (Saccharum officinarum L.) Nature 161: 608.
Price, S., 1957. Cytological studies in Saccharum and allied genera. III. Chromosome numbers in interspecific hybrids. Bot. Gaz. 118: 146–159.
Price, S., 1965. Interspecific hybridization in sugarcane breeding. Proc. Int. Soc. Sugar Cane Technol. 12: 1021–1026.
Sills, G.R., W. Bridges, S.M. Al-Janabi & B.W.S. Sobral, 1995. Genetic analysis of agronomic traits in a cross between sugarcane (Saccharum officinarum L.) and its presumed progenitor (S. robustum Brandes & Jesw. ex Grassl). Mol. Breed. 1: 355–363.
Sobral, B.W.S., D.P.V. Braga, E.S. Lahood & P. Keim, 1994. Phylogenetic analysis of chloroplast restriction enzyme site mutations in the Saccharinae Griseb. subtribe of the Andropogoneae Dumort. tribe. Theor. Appl. Genet. 87: 843–853.
Walbot, V., 1988. Preparation of DNA from single rice seedlings. Rice Genet. Newsl. 5: 149–151.
Warner, J.N., 1962. Sugarcane: An indigenous Papuan cultigen. Ethnology 1: 405–411.
Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V., Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531–6535.
Williams, M.N.V., N. Pande, S. Nair, M. Mohan & J. Bennett, 1991. Restriction fragment length polymorphism analysis of polymerase chain reaction products amplified from mapped loci of rice (Oryza sativa L.) genomic DNA. Theor. Appl. Genet. 82: 489–498.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nair, N.V., Nair, S., Sreenivasan, T. et al. Analysis of genetic diversity and phylogeny in Saccharum and related genera using RAPD markers. Genetic Resources and Crop Evolution 46, 73–79 (1999). https://doi.org/10.1023/A:1008696808645
Issue Date:
DOI: https://doi.org/10.1023/A:1008696808645