Summary
ATP (1–1.2mm) was photoreleased from caged ATP (5mm) in skinned fibres from rat psoas muscle at 15–17° C, to examine the effects of ethylene glycol (EG; 20% in solvent) on the kinetics of isometric contraction. Muscle fibres were stretched by 0.5–2% before photolysis, so that force just before photolysis was almost equal to the steady-state force after photolysis. At the phase of steady-state contraction, force and 500 Hz-stifmess in the presence of EG were 50% and 70% of the controls, respectively, resulting in a higher stiffness-to-force with EG, as reported previously. Following photolysis, force fell before rising to a steady-state plateau. The estimated rate constant of the force decay was approximately 90 s−1, and in the presence of EG was 80–85% of the control. This suggested a small effect of EG on the crossbridge detachment induced by ATP. The rate of force redevelopment was approximately 70 s−1, and EG decreased this rate to 50% of the control. This suggested that EG greatly slows the transition of the crossbridges from the detached state to the reattached force-producing state. The time course of the stiffness signals was consistent with this interpretation. The high stiffness-to-force ratio with EG indicated that EG not only reduces the rate constants which were directly examined in this study but also modifies other aspects of the crossbridge reaction.
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Horiuti, K., Sakoda, T., Takei, M. et al. Effects of ethylene glycol on the kinetics of contraction on flash photolysis of caged ATP in rat psoas muscle fibres. J Muscle Res Cell Motil 13, 199–205 (1992). https://doi.org/10.1007/BF01874157
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DOI: https://doi.org/10.1007/BF01874157