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A simple microtiter plate screening assay for bacterial invasion or adherence

  • Published:
Methods in Cell Science

Abstract

Many bacteriologic studies, including cellular invasion and adherence assays, require enumeration of viable organisms or colony forming units. When attempting to screen large numbers of clinical or environmental isolates or laboratory-derived mutants for differences in invasion or adherence phenotype, standard plating methods can be cumbersome and severely limit the number of organisms or conditions which can be tested. As a potential alternative, we describe a simple, rapid and inexpensive soft agar-based technique for semi-quantitative determination of bacterial colony counts directly within the wells of a 96-well microtiter plate.

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Authors and Affiliations

  1. Department of Pediatrics, University of California, San Diego, La Jolla, California, USA

    Victor Nizet

  2. Department of Molecular Microbiology & Immunology, University of Missouri-Columbia Medical School, Columbia, Missouri, USA

    Arnold L. Smith

  3. Department of Medicine, Veteran Affairs Medical Center and, University of California – San Francisco, San Francisco, California, USA

    Paul M. Sullam

  4. Division of Infectious Diseases, Children's Hospital & Medical Center, University of Washington, Seattle, Washington, USA

    Craig E. Rubens

Authors
  1. Victor Nizet
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  2. Arnold L. Smith
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  3. Paul M. Sullam
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  4. Craig E. Rubens
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Nizet, V., Smith, A.L., Sullam, P.M. et al. A simple microtiter plate screening assay for bacterial invasion or adherence. Methods Cell Sci 20, 107–111 (1998). https://doi.org/10.1023/A:1009830606819

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  • DOI: https://doi.org/10.1023/A:1009830606819

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