Summary
An enzyme electrophoresis method is described for determining and monitoring the species of cells in culture. Electrophoresis runs are completed in 25 min. The methods used are straightforward and do not require a large investment in preparation time or equipment.
Similar content being viewed by others
V. References
Stulberg, C. S. Contamination in tissue culture. Fogh, J. ed. Extrinsic cell contamination of tissue cultures. New York: Academic Press; 1973: 1–27.
Stulburg, C. S.; Peterson, W. D., Jr.; Simpson, W. F. Identification of cells in culture. Am. J. Hematol. 1: 237–242; 1976.
Hukku, B.; Halton, D. M.; Mally, M.; Peterson, W. D., Jr. Cell characterization by use of multiple genetic markers: Proceedings Second International Cell Culture Congress. Plenum Press. In press.
Nelson-Rees, W. A. The identification and monitoring of cell line specificity. Origin and natural history of cell lines. New York, NY: Alan R. Liss Inc. 1978: 25–79.
Halton, D. M.; Peterson, W. D., Jr.; Hukku, B. Cell culture quality control by rapid isoenzymatic characterization. In Vitro 19: 16–24; 1983.
Montes De Oca, F.; Macy, M. L.; Shannon, J. E. Isoenzyme characterization of animal cell cultures. Proc. Soc. Exp. Biol. Med. 132: 462–469; 1969.
O'Brien, S. J.; Shannon, J. E.; Gail, M. H. A molecular approach to the identification of human and animal cells in culture: isozyme and allozyme genetic signatures. In Vitro 16: 119–135; 1980.
Harris, H.; Hopkinson, D. A. Handbook of enzyme electrophoresis in human genetics. New York, Oxford: North-Holland Publishing Co.; 1978.
Peterson, W. D., Jr.; Stulburg, C. S.; Swanborg, N. K.; Robinson, A. R. Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc. Soc. Exp. Biol. Med. 12: 772–776; 1968.
Peterson, W. D.; Simpson, W. F.; Hukku, B. Cell characterization: monitoring for cell identification. Jakoby, W. B.; Pastan, I. H. eds. Methods in enzymology: cell culture. New York: Academic Press; 1979: 164–178.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ottenbreit, M.J., Halton, D.M. & Peterson, W.D. Rapid isoenzyme analysis of cell cultures by agarose Electrophoresis. Journal of Tissue Culture Methods 7, 107–110 (1982). https://doi.org/10.1007/BF01665952
Issue Date:
DOI: https://doi.org/10.1007/BF01665952