Summary
A simple procedure for the rapid preparation of virus isolates from cell culture for negative-contrast electron microscopy was devised. Using only conventional centrifugation steps (i.e. without ultracentrifugation), the procedure produced consistent, fine-quality preparations of a variety of virus types differing in size/shape and buoyant density.
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Skilling, D.E., Barlough, J.E., Berry, E.S. et al. A simple, rapid method for preparation of virus isolates from cell culture for electron microscopy. Journal of Tissue Culture Methods 9, 217–220 (1986). https://doi.org/10.1007/BF01665758
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DOI: https://doi.org/10.1007/BF01665758