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Protonated polynucleotide structures

14. Poly(dG). poly(dC) and its metastable form

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Abstract

The circular dichroism spectra of poly(dG). poly(dC) have been studied as a function of pH in 0.15 M NaCl solution. Acid titration to pH 2.5 showed two transitions, one around pH 5, the second below pH 3. These transitions are disproportionation reactions and are not due to the dissociation of the complexes. Alkaline back titration to neutrality showed only one step above pH 7, without appearance of the intermediate form. The hysteresis loop observed gave rise to a metastable (probably protonated) form at pH 7 which reverted to the neutral form upon heating or alkali treatment. In order to obtain again the metastable hysteretic form, a whole titration cycle to pH 2.5 had to be performed.

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Abbreviations

CD:

circular dichroism

References

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Authors and Affiliations

  1. Service de Biochimie, Centre d'Études Nucléaires de Saclay, F-91190, Gif-sur-Yvette, France

    Danielle Thiele, Marie-Thérèse Sarocchi & Wilhelm Guschlbauer

  2. Groupe de Biophysique Moléculaire, Département de Biologie, Centre d'Études Nucléaires de Saclay, F-91190, Gif-sur-Yvette, France

    Christian Marck

Authors
  1. Danielle Thiele
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  2. Marie-Thérèse Sarocchi
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  3. Wilhelm Guschlbauer
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  4. Christian Marck
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Dedicated to Prof. A Janke (Vienna) on his 85th birthday.

Part 13 is ref. [1].

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Thiele, D., Sarocchi, MT., Guschlbauer, W. et al. Protonated polynucleotide structures. Molecular Biology Reports 1, 149–154 (1973). https://doi.org/10.1007/BF00357155

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  • DOI: https://doi.org/10.1007/BF00357155

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