Abstract
It is generally accepted that in psoriasis there is an alteration of epidermal cell proliferation. It has been reported that an increased rate of thymidine incorporation into keratinocytes is found in the upper part of the hair follicle in involved skin, but this is not the case in the lower part. Here we show that cells from psoriatic hair follicles could be brought in culture under the same conditions as those of normal hair follicles. Cells, whether originating from the upper or lower part of the hair follicle sheath either from involved or uninvolved psoriatic skin, show a faster rate of outgrowth in the first days of culture. Moreover, a large number of psoriatic cells have an increased motility in the early stages of culture, as compared to control cells. These properties can no longer be observed after several days in culture. The activity of glucose-6-phosphate dehydrogenase known to be increased in psoriatic plaques is normal in hair follicles isolated from these plaques. Protein gel electrophoretic investigations showed that there is no difference in gel patterns between normal and psoriatic hair follicles.
In conclusion, the isolation of human hair follicles represents a simple method that allows psoriatic keratinocytes to be brought in culture and permits the study of certain aspects of the disease.
Similar content being viewed by others
References
FisherL. B. & WellsG. L., 1968. British Journal of Dermatology 80: 235–240.
FuchsE. & GreenH., 1980. Cell 19: 1033–1042.
FuchsE. & GreenH., 1981. Cell 25: 617–625.
HalprinK. M. & OhkawaraA., 1966. Journal of Investigative Dermatology 46: 51–69.
HukkelhovenM. W. A. C., VermorkenA. J. M. & BloemendalH., 1980. Preparative Biochemistry 10: 473–481.
KruegerG. G., 1981. In: R. L.Dobson & H.Thiers (eds.) Yearbook of Dermatology. Yearbook Medical Publishers, Chicago-London, pp. 13–70.
LaemmliU. K., 1970. Nature 227: 680–685.
MiguelA., RamonM., PetitpierreE., GoosC. M. A. A., Vermeesch-MarkslagA. M. G. & VermorkenA. J. M., 1983. Human Genetics 64: 576–579.
ShahradP. & MarksR., 1976. British Journal of Dermatology 94: 7–12.
VermorkenA. J. M., WeteringsP. J. J. M. & BloemendalH., 1978. Molecular Biology Reports 4: 211–216.
VermorkenA. J. M., WirtzP., SpierenburgG. Th., VanBennekomC. A., DeBruynC. H. M. M. & OeiT. L., 1979. Journal of Clinical Chemistry and Clinical Biochemistry 17: 325–329.
WeberK. & OsbornM., 1969. Journal of Biological Chemistry 244: 4406–4412.
WeteringsP. J. J. M., VermorkenA. J. M. & BloemendalH. 1981. British Journal of Dermatology 104: 1–5.
WeteringsP. J. J. M., VermorkenA. J. M. & BloemendalH., 1982. Experimental Cell Research 139: 439–443.
WyattE. H. & RiggottJ. M., 1981. British Journal of Dermatology 105: 349–350.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vermorken, A.J.M., Lenoir, M.C., Shroot, B. et al. Psoriatic hair follicle cells I. Biochemistry and behaviour in culture. Mol Biol Rep 10, 41–47 (1984). https://doi.org/10.1007/BF00775152
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00775152