Skip to main content
SpringerLink
Log in

Non-essential repeats in the promoter region of a Brassica rapa acyl carrier protein gene expressed in developing embryos

  • Update Section
  • Sequence
  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

A genomic clone of an acyl carrier protein gene (Bcg4-4) which is highly expressed in developing embryos of Brassica rapa was isolated and sequenced. The promoter and transcription terminator regions of Bcg4-4 were used to express a β-glucuronidase reporter gene in transgenic rapeseed. Deletion of repeated domains in the promoter region did not lower β-glucuronidase expression in seeds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Harwood JL: Fatty acid metabolism. Annu Rev Plant Physiol 39: 101–138 (1988).

    Google Scholar 

  2. Jefferson RA: Assaying chimeric genes in plants. The GUS gene fusion system. Plant Mol Biol Rep 5: 387–405 (1987).

    Google Scholar 

  3. McBride KE, Summerfelt KR: Improved binary vectors for Agrobacterium-mediated plant transformation. Plant Mol Biol 14: 269–276 (1990).

    Google Scholar 

  4. Post-Beittenmiller MA, Hlousek-Radojcic A, Ohlrogge JB: DNA sequence of a genomic clone encoding an Arabidopsis acyl carrier protein (ACP). Nucl Acids Res 17: 1777 (1989).

    Google Scholar 

  5. Radke SE, Andrews BM, Moloney MM, Crouch ML, Kridl JC, Knauf VC: Transformation of Brassica napus L. using Agrobacterium tumefaciens: developmentally regulated expression of a reintroduced napin gene. Theor Appl Genet 75: 685–694 (1988).

    Google Scholar 

  6. Rose RE, De Jesus CE, Moylan SL, Ridge NP, Scherer DE, Knauf VC: Nucleotide sequence encoding acyl carrier protein (ACP) from Brassica campestris seeds. Nucl Acids Res 15: 7197 (1987).

    Google Scholar 

  7. de Silva J, Loader NM, Jarman C, Windust JHC, Hughes SG, Safford R: The isolation and sequence analysis of two seed-expressed acyl carrier protein genes from Brassica napus. Plant Mol Biol 14: 537–548 (1990).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Calgene, Inc., 1920 Fifth Street, 95616, Davis, CA, USA

    Donna Scherer, David W. McCarter, Sharon E. Radke, Jean C. Kridl & Vic C. Knauf

  2. Life Science Research Center, Nippon Steel Corporation, 1618 Ida, Nakahara-ku, 211, Kawasaki, Japan

    Akira Sato

Authors
  1. Donna Scherer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Akira Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David W. McCarter
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sharon E. Radke
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jean C. Kridl
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Vic C. Knauf
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scherer, D., Sato, A., McCarter, D.W. et al. Non-essential repeats in the promoter region of a Brassica rapa acyl carrier protein gene expressed in developing embryos. Plant Mol Biol 18, 591–594 (1992). https://doi.org/10.1007/BF00040675

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00040675

Key words

Search

Navigation