Abstract
In an attempt to isolate a plant malonyl-coenzyme A:acyl carrier protein transacylase cDNA clone, by direct genetic selection in an Escherichia coli fabD mutant (LA2-89) with a maize cDNA expression library, a Zea mays cDNA clone encoding a GTP-binding protein of the ARF family was isolated. Complementation of a mutation affecting bacterial membrane lipid biosynthesis by a plant ARF protein, could indicate the existence of as yet unidentified bacterial equivalents of this ubiquitous eucaryotic GTP-binding protein.
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References
Ames GF: Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism. J Bact 95: 833–843 (1968).
de Boer PAJ, Crossley RE, Rothfield LI: The essential bacterial cell-division protein FtsZ is a GTPase. Nature 359: 254–256 (1992).
Brown HA, Gutowski S, Moomaw CR, Slaughter C, Sternweis PC: ADP-ribosylation factor, a small GTP-dependent regulatory protein stimulates phospholipase D activity. Cell 75: 1137–1144 (1993).
CronanJr. JE: Phospholipid alteration during growth of Escherichia coli. J Bact 95: 2054–2061 (1968).
DiRusso CC, Metzger AK, Heimert TL: Regulation of transcription of genes required for fatty acid transport and unsaturated fatty acid biosynthesis in Escherichia coli by FadR. Mol Microbiol 7: 311–322 (1993).
Frisch DA, Tommey AM, Gengenbach BG, Somers DA: Direct genetic selection of a maize cDNA for dihydrodipicolinate synthase in an Escherichia coli dapA-auxotroph. Mol Gen Gen 228: 287–293 (1991).
Gish W, States DJ: Identification of protein coding regions by database similarity search. Nature Genet 3: 266–272 (1993).
Gurr MI, James AT: Lipid Biochemistry; An Introduction. Chapman and Hall, London (1975).
Hall A: The cellular functions of small GTP-binding proteins. Science 249: 635–640 (1990).
Harder ME, Ladenson RC, Schimmel SD, Silbert DF: Mutants of Escherichia coli with temperature-sensitive malonyl coenzyme A-acyl carrier protein transacylase. J Biol Chem 249: 7468–7475 (1974).
Harwood JL, Walsh MC, Walker KA: Enzymes of fatty acid synthesis. Meth Plant Biochem 3: 193–217 (1990).
Kahn RA, Der DJ, Bokoch GM: The ras superfamily of GTP-binding proteins: guidelines on nomenclature. FASEB J 6: 2512–2513 (1992).
Kahn RA, Yucel JK, Malhotra V: ARF signaling: a potential role for phospholipase D in membrane traffic. Cell 75: 1045–1048 (1993).
Kaziro Y, Itoh H, Kozasa T, Nakafuko M, Satoh T: Structure and function of signal-transducing GTP-binding proteins. Annu Rev Biochem 60: 349–400 (1991).
Luirink J, ten Hagen-Jongman CM, van der Weijden CC, Oudega B, High S, Dobberstein B, Kusters R: An alternative protein targeting pathway in Escherichia coli: studies on the role of FtsY. EMBO J 13: 2289–2296 (1994).
RayChaudhuri D, Park JT: Escherichia coli cell-division gene ftsZ encodes a novel GTP-binding protein. Nature 359: 251–254 (1992).
Regad F, Bardet C, Tremousaygue D, Moisan A, Lescure B, Axelos M: cDNA cloning and expression of an Arabidopsis GTP-binding protein of the ARF family. FEBS Lett 316: 133–136 (1993).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Serafini T, Orci L, Amherdt M, Brunner M, Kahn RA, Rothman JE: ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein. Cell 67: 239–253 (1991).
Snustad DP, Hunsperger JP, Chereskin BM, Messing J: Maize glutamine synthetase cDNAs: isolation by direct genetic selection in Escherichia coli. Genetics 120: 1111–1124 (1988).
Terryn N, Van Montagu M, Inzé D: GTP-binding proteins in plants. Plant Mol Biol 22: 143–152 (1993).
VerwoertII GS, Verbree EC, van der Linden KH, Nijkamp HJJ, Stuitje AR: Cloning, nucleotide sequence, and expression of the Escherichia coli fabD gene, encoding malonyl Coenzyme A-acyl carrier protein transacylase. J Bacteriol 174: 2851–2857 (1992).
VerwoertII GS, Verhagen EF, van der Linden KH, Verbree EC, Nijkamp HJJ, Stuitje AR: Molecular characterization of an Escherichia coli mutant with a temperature-sensitive malonyl Coenzyme A-acyl carrier protein transacylase. FEBS Lett 348: 311–316 (1994).
Yu C-L, Tsai M-H, Stacey DW: Cellular ras activity and phospholipid metabolism. Cell 52: 63–71 (1988).
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Verwoert, I.I.G.S., Brown, A., Slabas, A.R. et al. A Zea mays GTP-binding protein of the ARF family complements an Escherichia coli mutant with a temperature-sensitive malonyl-coenzyme A:acyl carrier protein transacylase. Plant Mol Biol 27, 629–633 (1995). https://doi.org/10.1007/BF00019329
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DOI: https://doi.org/10.1007/BF00019329