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Chalcone synthase in rice (Oryza sativa L.): Detection of the CHS protein in seedlings and molecular mapping of the chs locus

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Abstract

The chalcone synthase is a key enzyme that catalyses the first dedicated reaction of the flavonoid pathway in higher plants. The chs gene and its protein product in rice has been investigated. The presence of a chalcone synthase (CHS) protein in rice seedlings and its developmental stage-specific expression has been demonstrated by western analysis. The chalcone synthase of rice was found to be immunologically similar to that of maize. A rice cDNA clone, Os-chs cDNA, encoding chalcone synthase, isolated from a leaf cDNA library of an indica rice variety Purpleputtu has been mapped to the centromeric region of chromosome 11 of rice. It was mapped between RFLP markers RG2 and RG103. RG2 is the nearest RFLP marker located at a genetic distance of 3.3 cM. Some segments of chromosome 11 of rice including chs locus are conserved on chromosome 4 of maize. The markers, including chs locus on chromosome 11 of rice are located, though not in the same order, on chromosome 4 of maize. Genetic analysis of purple pigmentation in two rice lines, Abhaya and Shyamala, used in the present mapping studies, indicated the involvement of three genes, one of which has been identified as a dominant inhibitor of leaf pigmentation. The Os-chs cDNA shows extensive sequence homology, both for DNA and protein (deduced), to that of maize, barley and also to different monocots and dicots.

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References

  1. Abenes MLP, Tabien RE, McCouch SR, Ikeda R, Ronald P, Khush GS Huang N: Orientation and integration of the classical and molecular genetic maps of chromosome 11 in rice. Euphytica 76: 81–87 (1994).

    Google Scholar 

  2. Ahn S, Tanksley SD: Comparative linkage maps of the rice and maize genomes. Proc Natl Acad Sci USA 90: 7980–7984 (1993).

    Google Scholar 

  3. Akada S, Dube SK: Organization of soybean chalcone synthase gene clusters and characterization of a new member of the family. Plant Mol Biol 29: 189–199 (1995).

    Google Scholar 

  4. Anonymous: In: Maize Genet Coop Newsl p. 250 (1995).

  5. Batschauer A, Ehmann B, Schafer E: Cloning and characterization of a chalcone synthase gene from mustard and its light dependent expression. Plant Mol Biol 16: 175–185 (1991).

    Google Scholar 

  6. Bonierbale M, Plaisted RL, Tanksley SD: RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120: 1095–1103 (1988).

    Google Scholar 

  7. Causse MA, Fulton TM, Cho YG, Ahn SN, Chunwongse J, Wu K, Xiao J, Yu Z, Ronald PC, Harrington SE, Second G, McCouch SR, Tanksley SD: Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 38: 1251–1274 (1994).

    Google Scholar 

  8. Coe EH, Neuffer MG, Hoisington DA: The genetics of corn. In: Sprague GF, Dudley JW (eds) Corn and Corn Improvement, pp. 81–258. American Society of Agronomy, Madison, WI (1988).

    Google Scholar 

  9. Coe EH, McCormick S, Modena SA: White pollen in maize. J Hered 72: 318–320 (1981).

    Google Scholar 

  10. Dangl JL, Hahlbrock K, Schell J: Regulation and structure of chalcone synthase genes. In: Vasil IK, Schell J (eds) Cell Culture and Somatic Cell Genetics of Plants, vol. 4, pp. 155–173. Academic Press, New York (1989).

    Google Scholar 

  11. Epping B, Kittel M, Ruhnau B, Hemleben V: Isolation and sequence analysis of a chalcone synthase cDNA of Matthiola incana R.Br (Brassicaceae). Plant Mol Biol 14: 1061–1063 (1990).

    Google Scholar 

  12. Feinbaum RL, Ausubel FM: Trancriptional regulation of the Arabidopsis thaliana chalcone synthase gene. Mol Cell Biol 8: 1985–1992 (1988).

    Google Scholar 

  13. Harker CL, Ellis THN, Coen ES: Identification and genetic regulation of chalcone synthase multigene family in pea. Plant Cell 2: 185–194 (1990).

    Google Scholar 

  14. Helariutta Y, Elomaa P, Kotilainen M, Griesbach RJ, Schroder J, Teeri TH: Chalcone synthase-like genes active during corolla development are differentially expressed and encode enzymes with different catalytic properties in Gerbera hybrida (Asteraceae). Plant Mol Biol 28: 47–60 (1995).

    Google Scholar 

  15. Heller W, Hahlbrock K: Highly purified flavanone synthase from parsley catalyses the formation of naringenin chalcone. Arch Biochem Biophys 200: 617–619 (1980).

    Google Scholar 

  16. Hulbert SH, Richter TE, Axtell JD, Bennetzen JL: Genetic mapping and characterization of sorghum and related crops by means of maize DNA probes. Proc Natl Acad Sci USA 87: 4251–4255 (1990).

    Google Scholar 

  17. Jahnen W, Hahlbrock K: Differential regulation and tissue specific distribution of enzymes of phenylpropoanoid pathways in developing parsley seedlings. Plant 173: 197–204 (1988).

    Google Scholar 

  18. Jorgensen R: Altered gene expression in plants due to trans interactions between homologous genes. Trends Biotechnol 8: 340–344 (1990).

    Google Scholar 

  19. Kilian A, Kudrna DA, Kleinhofs A, Yano M, Kurata N, Steffenson B, Sasaki T: Rice-barley synteny and its application to saturation mapping of the barley Rpg1 region. Nucl Acids Res 23: 2729–2733 (1995).

    Google Scholar 

  20. Koes RE, Spelt CE, Mol JNM, Gerats AGM: The chalcone synthase multigene family of Petunia hybrida (V30): sequence homology, chromosomal localization and evolutionary aspects. Plant Mol Biol 10: 159–169 (1987).

    Google Scholar 

  21. Koes RE, Spelt CE, van denElzen PJM, Mol JNM: Cloning and molecular characterization of the chalcone synthase multigene family of Petunia hybrida. Gene 81: 245–257 (1989).

    Google Scholar 

  22. Koes RE, vanBlokland R, Quattrocchio F, vanTunen AJ, Mol JNM: Chalcone synthase promoters in Petunia are active in pigmented and unpigmented cell types. Plant Cell 2: 379–392 (1990).

    Google Scholar 

  23. Kurata N, Moore G, Nagamura Y, Foote T, Yano M, Minobe Y, Gale M: Conservation of genome structure between rice and wheat. Bio/technology 12: 276–278 (1994).

    Google Scholar 

  24. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L: MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–181 (1987).

    Google Scholar 

  25. Lawton MA, Lamb CJ: Transcriptional activation of plant defense genes by fungal elicitor, wounding and infection. Mol Cell Biol 7: 335–341 (1987).

    Google Scholar 

  26. Lipphardt S, Brettschneider R, Kreuzaler F, Schell J, Dangl JL: UV-inducible transient expression in parsley protoplasts identifies regulatory cis-elements of a chimeric Antirrhinum majus chalcone synthase gene. EMBO J 7: 4027–4033 (1988).

    Google Scholar 

  27. McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD: Molecular mapping of rice chromosomes. Theor Appl Genet 76: 815–829 (1988).

    Google Scholar 

  28. McCouch SR, Tanksley SD: Development and use of restriction fragment length polymorphism in rice bereeding and genetics. In: Khush GS, Toenniessen GH (eds) Rice Biotechnology, pp. 109–133. CAB International, Wallingford, UK (1991).

    Google Scholar 

  29. Meier BM, Shaw N, Slusarenko AJ: Spatial and temporal accumulation of defense genes transcripts in bean (Phaseolus vulgaris) leaves in relation to bacteria-induced hypersensitive cell death. Mol Plant-Microb Interact 6: 453–466 (1993).

    Google Scholar 

  30. Mo Y, Nagel C, Taylor LP: Biochemical complementation of chalcone synthase mutants defines a role for flavonols in functional pollen. Proc Natl Acad Sci USA 89: 7213–7217 (1992).

    Google Scholar 

  31. Mohan M, Nair S, Bentur JS, Prasada Rao U, Bennett J: RFLP and RAPD mapping of the rice Gm2 gene that confers resistance to biotype 1 of gall midge (Orseolia oryzae). Theor Appl Genet 87: 782–788 (1994).

    Google Scholar 

  32. Moore G, Devos KM, Wang Z, Gale MD: Grasses, line up and form a circle. Curr Biol 5: 737–739 (1995).

    Google Scholar 

  33. Murray MG, Thompson WF: Rapid isolation of high molecular weight plant DNA. Nucl Acids Res 8: 4321–4325 (1980).

    Google Scholar 

  34. Nair S, Prasada Rao U, Bennett J, Mohan M: Detection of a highly heterozygous locus in recombinant inbred lines of rice and its possible involvement in heterosis. Theor Appl Genet 91: 978–986 (1995).

    Google Scholar 

  35. Napoli C, Lemieux C, Jorgensen R: Introduction of a chimeric chalcone synthase gene into Petunia results in reversible cosuppression of homologous genes in trans. Plant Cell 2: 279–289 (1990).

    Google Scholar 

  36. Niesbach-Klosgen U, Barzen E, Bernhardt J, Rohde W, Schwarz-Sommer Z, Reif HJ, Weinand U, Saedler H: Chalcone synthase genes in plants: a tool to study evolutionary relationships. J Mol Evol 26: 213–225 (1987).

    Google Scholar 

  37. Reddy AR: Genetics and molecular analysis of the anthocyanin pigmentation pathway in rice. In: Proceedings of the International Rice Genetics Meetings, Manila, Philippines, 15–21 October (1995).

  38. Reddy AR, Saedler AG, Scheffler B, Wienand U: Studies on transposable elements and anthocyanin pathway in rice: gene cloning and characterization. In: Proceedings of the International Program on Rice Biotechnology, Bali, Indonesia, 16–20 May, p. 155 (1994).

  39. Reddy VS, Dash S, Reddy AR: Andiocyanin pathway in rice (Oryza sativa L.): Identification of a mutant showing dominant inhibition of anthocyanins in leaf and accumulation of proanthocyanidins in pericarp. Theor Appl Genet 91: 301–312 (1995).

    Google Scholar 

  40. Reddy VS, Goud K, Sharma R, Reddy AR: Ultraviolet-B-responsive anthocyanin production in rice cultivar is associated with a specific phase of phenylalanine ammonia lyase biosynthesis. Plant Physiol 105: 1059–1066 (1994).

    Google Scholar 

  41. Reimold U, Kroeger M, Kreuzaler F, Hahlbrock K: Coding and 3′ non-coding nucleotide sequence of chalcone synthase mRNA and assignment of amino acid sequence of the enzyme. EMBO J 2: 1801–1805 (1983).

    Google Scholar 

  42. Ryder TB, Hedrick SA, Bell JN, Liang X, Clouse SD, Lamb CJ: Organization and differential activation of a gene family encoding the plant defense enzyme chalcone synthase in Phaseolus vulgaris. Mol Gen Genet 210: 219–233 (1987).

    Google Scholar 

  43. Scheffler BE, Reddy AR, Hoffman I, Wienand U: Chalcone synthase cDNA from Oryza sativa. Plant Physiol 109: 722 (1995).

    Google Scholar 

  44. Schmelzer E, Jahnen W, Hahlbrock K: In situ localization of light-induced chalcone synthase mRNA, chalcone synthase, and flavonoid end products in epidermal cells of parsley leaves. Proc Natl Acad Sci USA 85: 2989–2993 (1988).

    Google Scholar 

  45. Schmid J, Doerner PW, Clouse SD, Dixon RA, Lamb CJ: Developmental and environmental regulation of a bean chalcone synthase promoter in transgenic tobacco. Plant Cell 2: 619–631 (1990).

    Google Scholar 

  46. Schwarz-Sommer Z, Gierl A, Cuypers H, Peterson PA, Saedler H: Plant transposable elements generate the DNA sequence diversity needed in evolution. EMBO J 4: 591–597 (1985).

    Google Scholar 

  47. Sommer H, Saedler H: Structure of the chalcone synthase gene of Antirrhinum majus. Mol Gen Genet 202: 429–434 (1986).

    Google Scholar 

  48. Song YC, Gustafson JP: The physical location of fourteen RFLP markers in rice (Oryza sativa L.). Theor Appl Genet 90: 113–119 (1995).

    Google Scholar 

  49. Takahashi M: Genetical studies on rice. LXXX. Gene analysis and its related problems. J Fac Agr Hokkaido Univ 61: 91–142 (1982).

    Google Scholar 

  50. Tanksley SD, Ganal MW, Prince JP, deVicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Roder MS, Wing RA, Wu W, Young ND: High density molecular linkage maps of the tomato and potato genomes. Genetics 132: 1141–1160 (1992).

    Google Scholar 

  51. Taylor LP, Jorgensen R: Conditional male fertility in chalcone synthase deficient Petunia. J Hered 83: 11–17 (1992).

    Google Scholar 

  52. van derMeer IM, Spelt CE, Mol JNM, Stuitze AR: Promoter analysis of chalcone synthase (chsA) gene of Petunia hybrida: a 67 bp promoter region directs flower-specific expression. Plant Mol Biol 15: 95–109 (1990).

    Google Scholar 

  53. van derMeer IM, Stam ME, vanTunen AJ, Mol JNM, Stuitze AR: Antisense inhibition of flayonoid biosynthesis in Petunia anthers results in male sterility. Plant Cell 4: 253–262 (1992).

    Google Scholar 

  54. vanDeynze AE, Nelson JC, Yglesias ES, Harrington SE, Braga DP, McCouch SR, Sorrells ME: Comparative mapping in grasses. Wheat relationships. Mol Gen Genet 248: 744–754 (1995).

    Google Scholar 

  55. vanTunen AJ, Koes RE, Spelt CE, van derKrol AR, Stuitje AR, Mol JNM: Cloning of the two chalcone flavanone isomerase genes from Petunia hybrida: coordinate, light regulated and differential expression of flavonoid genes. EMBO J 7: 1257–1263 (1988).

    Google Scholar 

  56. Whitkus R, Doebley J, Lee M: Comparative genome mapping of sorghum and maize. Genetics 132: 1119–1130 (1992).

    Google Scholar 

  57. Wienand U, Weydemann U, Niesback-Klosgen U, Peterson PA, Saedler H: Molecular cloning of the c2 locus of Zea mays, the gene coding for chalcone synthase. Mol Gen Genet 203: 202–207 (1986).

    Google Scholar 

  58. Wingender R, Rohrig H, Horicke C, Wing D, Schell J: Differential regulation of soybean chalcone synthase genes in plant defense, symbiosis and upon environmental stimuli. Mol Gen Genet 218: 315–322 (1989).

    Google Scholar 

  59. Ylstra B, Busscher J, Franken J, Hollman PCH, Mol JNM, Tunen AJV: Flavonoids and fertilization in Petunia hybrida: localization and mode of action during pollen growth. Plant J 6: 201–212 (1994).

    Google Scholar 

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Authors and Affiliations

  1. International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110 067, New Delhi, India

    P. V. Sathyanarayanan, Suresh Nair & Madan Mohan

  2. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, 500 046, Hyderabad, India

    Arjula R. Reddy & G. Madhuri

  3. Algemeine Botanik, Plant Molecular Biology, University of Hamburg, Hamburg, Germany

    Brian Scheffler

  4. Department of Plant Breeding and Genetics, Indira Gandhi Agricultural University, Raipur, India

    M. N. Srivastava & Arvind Kumar

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  1. Arjula R. Reddy
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  2. Brian Scheffler
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  3. G. Madhuri
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  4. M. N. Srivastava
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  5. Arvind Kumar
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  6. P. V. Sathyanarayanan
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  7. Suresh Nair
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Reddy, A.R., Scheffler, B., Madhuri, G. et al. Chalcone synthase in rice (Oryza sativa L.): Detection of the CHS protein in seedlings and molecular mapping of the chs locus. Plant Mol Biol 32, 735–743 (1996). https://doi.org/10.1007/BF00020214

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  • DOI: https://doi.org/10.1007/BF00020214

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