Skip to main content
Log in

Survival and competitive ability of ammonia excreting and nonammonia excretingAzotobacter chroococcum strains in sterile soil

  • Published:
Plant and Soil Aims and scope Submit manuscript

Summary

The survival of two multiple antibiotic resistant native isolates ofAzotobacter chroococcum (A10 and A42), one of them (A10) having high nitrogenase activity and high ammonia excreting ability and another (A42) having low nitrogenase activity and low ammonia excreting ability were studied in sterile sandy loam soil with amendments of compost and glucose and without amendments. The decrease in cell numbers over a period of four weeks in all the three types of treatments approximated 4 log units. However, the death rate was more in the first two weeks. In all the treatments A10 showed better survival and competitive ability than A42 both singly and in mixed culture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Abd-el-Malek Y 1971 Free living nitrogen fixing bacteria in Egyptian soils and their possible contribution to soil fertility. Plant and Soil Spec. Vol. 423–442.

  2. Bishop P E, Gordon J K, Shah V K and Brill W J 1977 Transformation of nitrogen fixation genes in Azotobacter.In Genetic Engineering for Nitrogen Fixation. Ed. A Hollaender et al. Plenum Press, New York. pp 67–80.

    Google Scholar 

  3. Brown M E, Burlingham S K and Jackson R M 1962 Studies on Azotobacter sps in soil. I. Comparison of media and techniques for counting Azotobacter in soil. Plant and Soil 17, 309–319.

    Google Scholar 

  4. Burris R H 1974 Methodology.In The Biology of Nitrogen Fixation. Ed. A Quispel. North-Holland Publ. Co. Amsterdam. pp 9–33.

    Google Scholar 

  5. Cole M A 1977 Antibiotic resistance inRhizobium japonicum, implications for safety and interpretation of Nif transfer from Rhizobium.In Genetic Engineering for Nitrogen Fixation. Ed. A. Hollaender et al. Plenum Press, New York. pp 423–431.

    Google Scholar 

  6. Danso S K A and Alexander M 1974 Survival of two strains of Rhizobium in soil. Soil Sci. Soc. Am. Proc. 38, 86–89.

    Google Scholar 

  7. Dickey R S 1961 Relation of some edaphic factors toAgrobacterium tumefaciens. Phytopathology 51, 607–614.

    Google Scholar 

  8. Diem H G and Dommergues Y R 1979 Significance and improvement of rhizospheric nitrogen fixation.In Recent advances in Biological Nitrogen fixation. Ed. N S Subba Rao. Oxford and IBH Publishing Co., New Delhi. pp 190–226.

    Google Scholar 

  9. Döbereiner J 1974 Nitrogen fixing bacteria in the rhizosphere.In The Biology of Nitrogen Fixation. Ed. A Quispel. North-Holland Publishing Co. Amsterdam. pp 86–120.

    Google Scholar 

  10. Klein D A and Casida Jr L E 1967Escherichia coli die-out from normal soil as related to nutrient availability and the indigenous microflora. Can. J. Microbiol. 13, 1461–1470.

    Google Scholar 

  11. Narula N, Lakshminarayana K and Tauro P 1980 Field evaluation ofRhizobium japonicum and soybean varieties by acetylene reduction method. Indian J. Microbiol. 20, 298–301.

    Google Scholar 

  12. Narula N, Lakshminarayana K and Tauro P 1981 Ammonia excretion byAzotobacter chroococcum. Biotechn. Bioeng. 23, 467–470.

    Google Scholar 

  13. Satyavir Singh 1980 Studies on nitrogen fixation by free living organisms in Haryana Soils. M.Sc. Thesis. Haryana Agril. University, Hissar.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Singh, S., Lakshminarayana, K. Survival and competitive ability of ammonia excreting and nonammonia excretingAzotobacter chroococcum strains in sterile soil. Plant Soil 69, 79–84 (1982). https://doi.org/10.1007/BF02185706

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02185706

Key words

Navigation