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Molecular cloning of the plant plasma membrane H+-ATPase

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Summary

Mineral transport across the plasma membrane of plant cells is controlled by an electrochemical gradient of protons. This gradient is generated by an ATP-consuming enzyme in the membrane known as a proton pump, or H+-ATPase. The protein has a catalytic subunit of Mr=100,000 and is a prominent band when plasma membrane proteins are analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

We generated specific rabbit polyclonal antibody against the Mr=100,000 H+-ATPase and used the antibody to screen λgtll expression vector libraries of plant DNA. Several phage clones producing immunoreactive protein, and presumably containing DNA sequences for the ATPase structural gene, were isolated and purified from a carrot cDNA library and a Arabidopsis genomic DNA library. These studies represent our first efforts at cloning the structural gene for a plant plasma membrane transport protein. Applicability of the technique to other transport protein genes and the potential for use of recombinant DNA technology in plant mineral transport research are discussed.

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Authors and Affiliations

  1. Department of Horticulture, University of Wisconsin, 53706, Madison, WI, USA

    T. K. Surowy & M. R. Sussman

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  1. T. K. Surowy
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  2. M. R. Sussman
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Surowy, T.K., Sussman, M.R. Molecular cloning of the plant plasma membrane H+-ATPase. Plant Soil 99, 185–196 (1987). https://doi.org/10.1007/BF02370165

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