Skip to main content
SpringerLink
Log in

Changes in composition of membrane proteins accompanying the regulation of PS I/PS II stoichiometry observed with Synechocystis PCC 6803

  • Regular Paper
  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

Changes in composition of membrane proteins in Synechocystis PCC 6803 induced by the shift of light regime for photosynthetic growth were studied in relation to the regulation of PS I/PS II stoichiometry. Special attention was paid to the changes in abundance of proteins of PS I and PS II complexes. Composition was examined using a LDS-PAGE and a quantitative enzyme immunoassay. Abundance of PsaA/B polypeptides and the PsaC polypeptide of the PS I complex, on a per cell basis, increased under the light regime exciting preferentially PS II and decreased under the light regime exciting mainly PS I. Similar changes were observed with polypeptides of 18.5, 10 and 8.5 kDa. The abundance of other proteins associated with membranes, including PsbA polypeptide of the PS II complex, was fairly constant irrespective of light regime. These results are consistent with our previous observations with other strains of cyanophytes (Anabaena variabilis M2 and Synechocystis PCC 6714) that PS I is the variable component in changes in PS I/PS II stoichiometry in response to changing light regimes for photosynthesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CBB:

Coomassie brilliant blue

Chl:

chlorophyll

EIA:

enzyme immunoassay

LDS:

lithium dodecyl sulfate

PAGE:

polyacrylamide gel electrophoresis

PS:

photosystem

PVDF:

polyvinylidene difluoride

References

  • Alhadeff M, Lundell DJ and Glazer AN (1988) Structural studies on cyanobacterial Photosystem I. Purification and characterization of two low molecular weight polypeptides. Arch Microbiol 150: 482–488

    Google Scholar 

  • Brown RE, Jarvis KL and Hyland KJ (1989) Protein measurement using bicinchoninic acid: Elimination of interfering substances. Anal Biochem 180: 136–139

    PubMed  Google Scholar 

  • Chitnis PR, Reilly PA, Miedel MC and Nelson N (1989) Structure and targeted mutagenesis of the gene encoding 8-kDa subunit of Photosystem I from the cyanobacterium Synechocystis sp. PCC 6803. J Biol Chem 264: 18374–18380

    PubMed  Google Scholar 

  • Chitnis PR, Purvis D and Nelson N (1991) Molecular cloning and targeted mutagenesis of the gene psaF encoding subunit III of Photosystem I from the cyanobactierum Synechocystis sp. PCC 6803. J Biol Chem 266: 20146–20151

    Google Scholar 

  • Chow WS, Melis A and Anderson JM (1990) Adjustments of photosystems stoichiometry in chloroplasts improve the quantum efficiency of photosynthesis. Proc Natl Acad Sci USA 87: 7502–7506

    PubMed  Google Scholar 

  • Cunningham FXJr, Dennenberg RJ, Jursinic PA and Gantt E (1990) Growth under red light enhances Photosystem II relative to Photosystem I and phycobilisomes in the red alga Porphyridium cruentum. Plant Physiol 93: 888–895

    Google Scholar 

  • Delepelaire P and Chua N-H (1979) Lithium dodecyl sulfate/polyacrylamide gel electrophoresis of thylakoid membranes at 4°C: Characterizations of two additional chlorophyll a-protein complexes. Proc Natl Acad Sci USA 76: 111–115

    Google Scholar 

  • Domingo A and Marco R (1989) Visualization under ultraviolet light enhances 100-fold the sensitivity of peroxidase-stained blots. Anal Biochem 182: 176–181

    PubMed  Google Scholar 

  • Eichacker LA, Soll J, Lauterbach P, Rudiger W, Klein RR and Mullet JE (1990) In vitro synthesis of chlorophyll A in the dark triggers accumulation of chlorophyll A apoproteins in barley etioplasts. J Biol Chem 265: 13566–13571

    PubMed  Google Scholar 

  • Franzén L-G, Frank G, Zuber H and Rochaix J-D (1989) Isolation and characterization of cDNA clones encoding the 17.9 and 8.1 kDa subunits of Photosystem I from Chlamydomonas reinhardtii. Plant Mol Biol 12: 463–474

    Google Scholar 

  • Fujita Y and Murakami A (1987) Regulation of electron transport composition in cyanobacterial photosynthetic system: Stoichiometry among Photosystem I and II complexes and their light-harvesting antennae and cytochrome b 6 f complex. Plant Cell Physiol 28: 1547–1553

    Google Scholar 

  • Fujita Y, Ohki K and Murakami A (1985) Chromatic regulation of photosystem composition in the photosynthetic system of red and blue-green algae. Plant Cell Physiol 26: 1541–1548

    Google Scholar 

  • Fujita Y, Ohki K and Murakami A (1987a) Chromatic regulation of photosystem composition in the cyanobacterial photosynthetic system: Kinetic relationship between change of photosystem composition and cell proliferation. Plant Cell Physiol 28: 227–234

    Google Scholar 

  • Fujita Y, Murakami A and Ohki K (1987b) Regulation of photosystem composition in the cyanobacterial photosynthetic system: the regulation occurs in response to the redox state of the electron pool located between the two photosystems. Plant Cell Physiol 28: 283–292

    Google Scholar 

  • Fujita Y, Murakami A, Ohki K and Hagiwara N (1988) Regulation of photosystem composition in cyanobacterial photosynthetic system: Evidence indicating that Photosystem I formation is controlled in response to the electron transport state. Plant Cell Physiol 29: 557–564

    Google Scholar 

  • Fujita Y, Murakami A and Ohki K (1990) Regulation of the stoichiometry of thylakoid components in the photosynthetic system of cyanophytes: Model experiments showing that control of the synthesis or supply of Chl a can change the stoichiometric relationship between the two photosystems. Plant Cell Physiol 31: 145–153

    Google Scholar 

  • Garewal HS and Wasserman AR (1974) Triton X-100–4M urea as an extraction medium for membrane proteins. I. Purification of chloroplast cytochrome b 559. Biochemistry 13: 4063–4071

    PubMed  Google Scholar 

  • Hiyama T and Ke B (1972) Difference spectra and excitation coefficient of P700. Biochim Biophys Acta 267: 160–171

    PubMed  Google Scholar 

  • Johanningmeier U (1987) Expression of the psbA gene in E. coli. Z Naturforsch 42c: 755–757

    Google Scholar 

  • Kawamura M, Mimuro M and Fujita Y (1979) Quantitative relationship between two reaction centers in the photosynthetic system of blue-green algae. Plant Cell Physiol 20: 697–705

    Google Scholar 

  • Klein RR, Gamble PE and Mullet JE (1988) Light-dependent accumulation of radiolabeled plastid-encoded chlorophyll a-apoproteins requires chlorophyll a I. Analysis of chlorophyll-deficient mutants and phytochrome involvement. Plant Physiol 88: 1246–1256

    Google Scholar 

  • Koike H, Ikeuchi M, Hiyama T and Inoue Y (1989) Identification of Photosystem I components from the cyanobacterium, Synechococcus vulcanus by N-terminal sequencing. FEBS Lett 253: 257–263

    Article  PubMed  Google Scholar 

  • Ley AC (1984) Effective absorption cross-sections in Porphyridium cruentum. Implications for energy transfer between phycobilisomes and Photosystem II reaction centers. Plant physiol 74: 451–454

    Google Scholar 

  • Mackinney G (1941) Absorption of light by chlorophyll solutions. J Biol Chem 140: 315–322

    Google Scholar 

  • Manodori A and Melis A (1986a) Light-quality regulates photosystem stoichiometry in cyanobacteria. In: Akoyunoglou G and Senger H (eds) Regulation of Chloroplast Differentiation, pp 653–662. Alan R. Liss Inc., New York

    Google Scholar 

  • Manodori A and Melis A (1986b) Cyanobacterial acclimation to Photosystem I or Photosystem II light. Plant Physiol 82: 185–189

    Google Scholar 

  • Marder JB, Mattoo AK and Edelman M (1986) Identification and characterization of the psbA gene product: The 32-kDa chloroplast membrane protein. Methods in Enzymol 118: 384–396

    Google Scholar 

  • Mattoo AK, Marder JB and Edelman M (1989) Dynamics of the Photosystem II reaction center. Cell 56: 241–246

    Article  PubMed  Google Scholar 

  • Melis A, Mullineaux and Alien JF (1989) Acclimation of the photosynthetic apparatus to Photosystem I or Photosystem II light: Evidence from quantum yield measurements and fluorescence spectroscopy of cyanobacterial cells. Z Naturforsh 44c: 109–118

    Google Scholar 

  • Murakami A and Fujita Y (1988) Steady state of photosynthesis in cyanobacterial photosynthetic systems before and after regulation of electron transport composition: Overall rate of photosynthesis and PS I/PS II composition. Plant Cell Physiol 29: 305–311

    Google Scholar 

  • Murakami A and Fujita Y (1991) Steady state of photosynthetic electron transport in cells of the cyanophyte Synechocystis PCC 6714 having different stoichiometry between PS I and PS II: Analysis of flash-induced oxidation-reduction of cytochrome f and P700 under steady state of photosynthesis. Plant Cell Physiol 32: 213–222

    Google Scholar 

  • Myers J, Graham J-R and Wang RT (1978) On spectral control of pigmentation in Anacystis nidulans (Cyanophyceae). J. Phycol 14: 513–518

    Google Scholar 

  • Myers J, Graham J-R and Wang RT (1980) Light harvesting in Anacystis nidulans studied in pigment mutants. Plant Physiol 66: 1144–1149

    Google Scholar 

  • Ohki K, Okabe Y, Murakami A and Fujita Y (1987) A comparative study of quantitative relationships between phycobiliproteins and Photosystem II in cyanobacteria and red algae. Plant Cell Physiol 28: 1219–1226

    Google Scholar 

  • Reilly P, Hulmes JD, Pan Y-CE and Nelson N (1988) Molecular cloning and sequencing of the psaD gene encoding subunit II of Photosystem I from the cyanobacterium, Synechocystis sp. PCC 6803. J Biol Chem 263: 17658–17662

    PubMed  Google Scholar 

  • Takabe T, Takabe T and Akazawa T (1986) Biosynthesis of P700-chlorophyll a protein complex, plastocyanin, and cytochrome b 6/f complex. Plant Physiol 81: 60–66

    Google Scholar 

  • Watanabe A (1960) List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J Gen Appl Microbiol 6: 283–292

    Google Scholar 

  • Wynn RM and Malkin R (1988) Interaction of plastocyanin with Photosystem I: A chemical cross-linking study of the polypeptide that binds plastocyanin. Biochemistry 27: 5863–5869

    PubMed  Google Scholar 

  • Zhao J, Warren PV, Li N, Bryant DA and Golbeck JH (1990) Reconstitution of electron transport in Photosystem I with PsaC and PsaD proteins expressed in Escherichia coli. FEBS Lett 276: 175–180

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute for Basic Biology, Myodaiji, 444, Okazaki, Japan

    Katsunori Aizawa

  2. Department of Biochemistry, Faculty of Science, Saitama University, 338, Urawa, Japan

    Tokurou Shimizu & Tetsuo Hiyama

  3. Department of Biology, Faculty of Science, Okayama University, 700, Okayama, Japan

    Kimiyuki Satoh

  4. Bioresearch Laboratory, Toyota Motor Co, 471, Toyota, Aichi, Japan

    Yohko Nakamura

Authors
  1. Katsunori Aizawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tokurou Shimizu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tetsuo Hiyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kimiyuki Satoh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yohko Nakamura
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aizawa, K., Shimizu, T., Hiyama, T. et al. Changes in composition of membrane proteins accompanying the regulation of PS I/PS II stoichiometry observed with Synechocystis PCC 6803. Photosynth Res 32, 131–138 (1992). https://doi.org/10.1007/BF00035947

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00035947

Key words

Search

Navigation