Abstract
Growth and differentiation of plant cell cultures was increased when media were gelled with corn starch instead of agar. Dry weight of tobacco and wild carrot cell cultures on media gelled with starch was more than three times that of cultures on media gelled with agar. Higher yield of anthocyanin and dry weight of embryos were found in wild carrot cultures grown on media gelled with corn starch. The starch-mediated increase in growth and differentiation of wild carrot cells was accompanied by an increase in density of the cultures shown by higher dry weight/fresh weight ratios.
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References
Arnold SV, Ericksson T (1984) Effect of agar concentrations on growth and anatomy of adventitous shoots of Picea abies (L) Karst. Plant Cell Tissue Organ Culture 3: 257–264
Singha S (1980) Influence of two commercial agars on in vitro shoot proliferation of ‘almey’ crab apple and ‘seckel’ pear. Hort Sci 19: 227–228
Debergh PC (1983) Effects of agar brand and concentration on the tissue culture media. Physiol Plantarum 59: 270–276
Kohlenbach H, Wernicke W (1978) Investigations on the inhibitory effect of agar and the function of active carbon in anther culture. Z Pflanzen Physiol 86: 463–472
Sorvari S (1986) The effect of starch gelatinized nutrient media in barley anther cultures. Ann Agric Fenn 25: 127–133
Sorvari S (1986) Differentiation of potato tuber discs in barley starch gelatinized nutrient media. Ann Agric Fenn 25: 135–138
Sorvari S (1986) Comparison of anther cultures of barley cultivars in barley-starch and agar gelatinized media. Ann Agric Fenn 25: 249–254
Kinnersley AM, Henderson WE (1988) Alternative carbohydrates promote differentiation of plant cells. Plant Cell Tissue and Organ Culture (in press)
Kinnersley AM, Dougall DK (1980) Increase in anthocyanin yield from wild-carrot cell cultures by a selection system based on cell aggregate size. Planta 149: 200–204
Verma DA, Dougall DK (1977) Influence of carbohydrates on quantitative aspects of growth and embryo formation in wild carrot suspension cultures. Plant Physiol 44: 1734–1737
Shuler ML (1981) Production of secondary metabolites from plant tissue culture-Problems and prospects. Ann New York Academy of Sciences 369: 65–80
Yeoman MM, Lindsey K, Miedzybrodyka MB, McLauchlan WR (1981) Accumulation of secondary products as a facet of differentiation in plant cell and tissue culture. In: Differentiation In Vitro, pp 65–85. 4th Symposium British Council of Cell Biology. Cambridge University Press, Cambridge
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Henderson, W.E., Kinnersley, A.M. Corn starch as an alternative gelling agent for plant tissue culture. Plant Cell Tiss Organ Cult 15, 17–22 (1988). https://doi.org/10.1007/BF00039885
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DOI: https://doi.org/10.1007/BF00039885