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Wheat anther culture: effect of genotype and environmental conditions

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Abstract

Twenty-two cultivars and lines of winter and spring wheat (Triticum aestivum L.) were studied, most for the first time, for their anther culture response. The response was genotype dependent. Plants grown in the field gave higher callus induction frequency than those grown in the greenhouse and the controlled environment chamber. Donor plants grown in a season of low drought stress as compared to a season of severe drought stress resulted in a higher frequency of callus induction. Spherical microcalli were observed in two wheat genotypes in some of only those anthers that were placed with only one loculus in contact with the medium. Wheat lines that were more responsive to anther culture were identified.

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Authors and Affiliations

  1. Department of Agronomy, Purdue University, 47907, W. Lafayette, IN, USA

    C. S. Lu, H. C. Sharma & H. W. Ohm

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  1. C. S. Lu
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  2. H. C. Sharma
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  3. H. W. Ohm
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Lu, C.S., Sharma, H.C. & Ohm, H.W. Wheat anther culture: effect of genotype and environmental conditions. Plant Cell Tiss Organ Cult 24, 233–236 (1991). https://doi.org/10.1007/BF00033482

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  • DOI: https://doi.org/10.1007/BF00033482

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