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Biosynthesis and accumulation of rosmarinic acid in suspension cultures ofColeus blumei

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Abstract

This communication reviews data on the accumulation and biosynthesis of rosmarinic acid in cell suspension cultures ofColeus blumei. The influence of the medium, mainly the carbohydrate source on growth and rosmarinic acid production in these cell cultures is described. The biosynthetic pathway of rosmarinic acid was elucidated inColeus blumei cell cultures: eight enzymatic activities are involved in the transformation of the precursors phenylalanine and tyrosine to the end product rosmarinic acid.

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Abbreviations

CAH:

cinnamic acid 4-hydroxylase

4CL:

4-coumarate:CoA ligase

HPPR:

hydroxyphenylpyruvate reductase

3-H:

hydroxycinnamoyl-hydroxyphenyllactate 3-hydroxylase

3′-H:

hydroxycinnamoyl-hydroxyphenyllactate 3′-hydroxylase

PAL:

phenylalanine ammonia-lyase

RAS:

rosmarinic acid synthase (hydroxycinnamoyl-CoA:hydroxyphenyllactate hydroxycinnamoyl transferase)

TAT:

tyrosine aminotransferase

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Authors and Affiliations

  1. Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, D-40225, Düsseldorf, Germany

    Maike Petersen, Elisabeth Szabo, Juliane Meinhard, Barbara Karwatzki, Claudia Gertlowski, Bettina Kempin & Elisabeth Fuß

Authors
  1. Maike Petersen
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  2. Elisabeth Szabo
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  3. Juliane Meinhard
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  4. Barbara Karwatzki
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  5. Claudia Gertlowski
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  6. Bettina Kempin
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  7. Elisabeth Fuß
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Petersen, M., Szabo, E., Meinhard, J. et al. Biosynthesis and accumulation of rosmarinic acid in suspension cultures ofColeus blumei . Plant Cell Tiss Organ Cult 43, 89–92 (1995). https://doi.org/10.1007/BF00052161

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