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Plant regeneration from protoplasts of the monocotyledonous Haworthia magnifica v. Poelln.

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Abstract

Calli were initiated from flower buds, gynoecia and inflorescence segments of Haworthia magnifica v. Poelln. and subcultured on solid medium. Two liquid culture steps were necessary to prepare the calli for the isolation of protoplasts capable of sustained cell divisions. Plants were regenerated from protoplast-derived calli. The influence of both the osmolality of the culture media and exudates on the viability of protoplasts and protoplast-derived cell colonies is briefly discussed.

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References

  1. Binding H (1974) Regeneration von haploiden und diploiden Pflanzen aus Protoplasten von Petunia hybrida L. Z Pflanzenphysiol 74:327–356

    Google Scholar 

  2. Beyl CA, Sharma GC (1983) Picloram induced somatic embryogenesis in Gasteria and Haworthia. Plant Cell Tissue Organ Culture 2:123–132

    Google Scholar 

  3. Bui-Dang-Ha D, Mackenzie IA (1973) The division of protoplasts from Asparagus officinalis L. and their growth and differentiation. Protoplasma 78:215–221

    Google Scholar 

  4. Durand J, Potrykus J, Donn G (1973) Plantes issues de protoplastes de Petunia. Z Pflanzenphysiol 69:26–34

    Google Scholar 

  5. Fitter MS, Krikorian AD (1981) Recovery of totipotent cells and plantlet production from daylily protoplasts. Ann Bot 48:591–597

    Google Scholar 

  6. Glimelius K (1984) High growth rate and regeneration capacity of hypocotyl protoplasts in some Brassicaceae. Physiol Plant 61:38–44

    Google Scholar 

  7. Kao KN, Gamborg OL, Michayluk MR, Keller MR, Miller RH (1973) The effect of sugars and inorganic salts on cell regeneration and sustained division in plant protoplasts. Colloq Int CNRS 212:207–213

    Google Scholar 

  8. Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105–110

    Google Scholar 

  9. Kaul K, Sabharwal PS (1972) Morphogenetic studies on Haworthia: establishment of tissue culture and control of differentiation. Am J Bot 59(4):377–385

    Google Scholar 

  10. Kaul K, Sabharwal PS (1975) Morphogenetic studies on Haworthia: effects of inositol on growth and differentiation. Am J Bot 62(6):655–659

    Google Scholar 

  11. Kohlenbach HW, Wenzel G, Hoffman F (1982) Regeneration of Brassica napus plantlets in cultures from isolated protoplasts of haploid stem embryos as compared with leaf protoplasts. Z Pflanzenphysiol 105:131–142

    Google Scholar 

  12. Lu CY, Vasil V, Vasil IK (1981) Isolation and culture of protoplasts of Panicum maximum Jacq. (Guinea grass): Somatic embryogenesis and plantlet formation. Z Pflanzenphysiol 104:311–318

    Google Scholar 

  13. Majumdar SK (1970) Culture of Haworthia inflorescences in vitro. J S Afr Bot 36(2):63–68

    Google Scholar 

  14. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Google Scholar 

  15. Ogihara Y (1979) Tissue culture in Haworthia. II. Effects of three auxins and kinetin on greening and redifferentiation of calluses. Bot Mag Tokyo 92:163–171

    Google Scholar 

  16. Ogihara Y, Tsunewaki K (1978) Tissue culture in Haworthia. I. Effects of auxins and kinetin on callus growth. Bot Mag Tokyo 91:83–91

    Google Scholar 

  17. Santos dos AVP, Outka DE, Cocking EC, Davey MR (1980) Organogenesis and somatic enbryogenesis in tissues derived from leaf protoplasts and leaf explants of Medicago sativa. Z Planzenphysiol 99:261–270

    Google Scholar 

  18. Schenck HR, Röbbelen G (1982) Somatic hybrids by fusion of protoplasts from Brassica oleracea and B. campestris. Z Pflanzenzüchtg 89:278–288

    Google Scholar 

  19. Vasil V, Vasil IK (1980) Isolation and culture of cereal protoplasts. II. Embryogenesis and plantlet formation from protoplasts of Pennisetum americanum. Theoret Appl Genet 56:97–99

    Google Scholar 

  20. Vasil V, Wang DY, Vasil IK (1983) Plant regeneration from protoplasts of Napier grass (Pennisetum purpureum Schum.). Z Pflanzenphysiol 111:233–239

    Google Scholar 

  21. Wessels DCJ, Groenewald EG, Koeleman A (1976) Callus formation and subsequent shoot and root development from leaf tissue of Haworthia planifolia var. cf. var. setulifera v.Poelln. Z Pflanzenphysiol 78:141–145

    Google Scholar 

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Author notes

  1. Yongru Sun

    Present address: Institute of Genetics, Academia Sinica, Beijing

Authors and Affiliations

  1. Botanisches Institut der Johann Wolfgang Goethe-Universität, Siesmayerstraße 70, D-6000, Frankfurt am Main, FRG

    Yongru Sun, Berthold M. Heil, Günter Kahl & Hans Willy Kohlenbach

Authors
  1. Yongru Sun
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  2. Berthold M. Heil
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  3. Günter Kahl
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  4. Hans Willy Kohlenbach
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Sun, Y., Heil, B.M., Kahl, G. et al. Plant regeneration from protoplasts of the monocotyledonous Haworthia magnifica v. Poelln.. Plant Cell Tiss Organ Cult 8, 91–100 (1987). https://doi.org/10.1007/BF00040736

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  • DOI: https://doi.org/10.1007/BF00040736

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