Abstract
The hepatic changes were compared in lambs photosensitized or not photosensitized after exposure to sporidesmin. Injury to both the parenchyma and the biliary system was more severe in the photosensitized than in the non-photosensitized lambs. The activities of γ-glutamyltransferase and glutamate dehydrogenase, and the total, conjugated and unconjugated bilirubin concentrations were significantly higher in sera from the photosensitized than from the non-photosensitized lambs. Hepatic glycogen levels were decreased in both the photosensitized and the non-photosensitized lambs, but were significantly lower in the former. Hence it is possible that lesions in hepatocytes contribute to retention of phylloerythrin and so to photosensitization.
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Anonymous, 1970. Empfehlungen der Deutschen Gesellschaft für Klinische Chemie. Standardisierung von Methoden zur Bestimmung von Enzymaktivitäten in biologischen Flüssigkeiten.Zeitschrift für Klinische Chemie und Klinische Biochemie,8, 658–660
Anonymous, 1971. Mitteilung der Deutschen Gesellschaft für Klinische Chemie. Festlegung einer einheitlichen Meßtemperatur für Enzymaktivitätsbestimmungen in der Klinischen Chemie.Zeitschrift für Klinische Chemie und Klinische Biochemie,9, 464–465
Anonymous, 1972. Empfehlungen der Deutschen Gesellschaft für Klinische Chemie. Standardisierung von Methoden zur Bestimmung von Enzymaktivitäten in biologischen Flüssigkeiten.Zeitschrift für Klinische Chemie und Klinische Biochemie,10, 182–192
Anonymous, 1974. Empfehlungen der Deutschen Gesellschaft für Klinische Chemie. Standardisierung von Methoden zur Bestimmung von Enzymaktivitäten in biologischen Flüssigkeiten.Zeitschrift für Klinische Chemie und Klinische Biochemie,12, 391
Bhattacharyya, G.K. and Johnson, R.A., 1977.Statistical Concepts and Methods, (John Wiley, New York)
Chowdhury, J.R., Wolkoff, A.W. and Arias, I.M., 1988. Heme and bile pigment metabolism. In: I.M. Arias, W.B. Jakoby, H. Popper, D. Schachter and D.A. Shafritz (eds),The Liver. Biology and Pathobiology, 2nd edn. (Raven Press, New York), 419–449
Cornelius, C.E., 1980. Liver function. In: J.J. Kaneko (ed.),Clinical Biochemistry of Domestic Animals, 3rd edn, (Academic Press, New York), 201–257
Ellis, T., Bradley, A., Watson, F., Elliott, K., Smith, G., McGrath, M. and Dolling, M., 1985. Protection of recently shorn sheep against adverse weather using plastic coats.Australian Veterinary Journal,62, 213–218
Flåøyen, A., 1990. Hepatogen fotosensibilitet hos sau [Hepatogenous photosensitivity diseases in sheep].Norsk Veterinœrtidsskrift,102, 183–190
Flåøyen, A., Borrebæk, B. and Nordstoga, K., 1991. Glycogen accumulation and histological changes in the livers of lambs with alveld and experimental sporidesmin intoxication.Veterinary Research Communications,15, 443–453
Jendrassik, L. and Gròf, P., 1938. Vereinfachte photometrische Methoden zur Bestimmung des Blutbilirubins.Biochemische Zeitschrift,297, 81–89
Keppler, D. and Decker, K., 1974. Glycogen. Determination with amyloglucosidase. In: H.U. Bergmeyer (ed.),Methods of Enzymatic Analysis, 2nd edn, (Academic Press, New York), 1127–1131
Klinger, W., Devereux, T. and Fouts, J.R., 1988. Functional and structural zonal hepatocyte heterogeneity-dynamics and ontogenic development.Experimental Pathology,35, 69–91
Mortimer, P.H., 1963. The experimental intoxication of sheep with sporidesmin, a metabolic product ofPithomyces chartarum. IV — Histological and histochemical examinations of orally-dosed sheep.Research in Veterinary Science,4, 166–185
Möwis, G., 1978.Histopatologisk Teknik, (Almqvist & Wiksell, Förlag, Stockholm), 145–147, 195
Oldman, L. and di Menna, M.E., 1983. Facial eczema. Predicting danger periods by spore counting.Aglink 4/6000/1/83: FPP 494, (MAF, Hamilton, New Zealand)
Oldman, L. and di Menna, M.E., 1985. Facial eczema. Fungicide control. Spraying techniques.Aglink FPP: 495:7/3000/10/1985, (MAF, Hamilton, New Zealand)
Pass, M.A. and Heath, T., 1977. Gallbladder paralysis in sheep during lantana poisoning.Journal of Comparative Pathology,87, 301–306
Persijn, J.P. and van der Silk, W., 1976. A new method for the determination of γ-glutamyltransferase in serum.Journal of Clinical Chemistry and Clinical Biochemistry,14, 421–427
Reichen, J. and Simon, F.R., 1988. Cholestasis. In: I.M. Arias, W.B. Jakoby, H. Popper, D. Schachter and D.A. Shafritz (eds),The Liver. Biology and Pathobiology, 2nd edn, (Raven Press, New York), 1105–1124
Smith, B.L. and Embling, P.P., 1991. Facial eczema in goats: the toxicity of sporidesmin in goats and its pathology.New Zealand Veterinary Journal,39, 18–22
Smith, B.L. and Payne, E., 1991. Adrenal-associated changes in experimental sporidesmin poisoning of sheep.New Zealand Veterinary Journal,39, 46–49
Szasz, G., Weimann, G., Stähler, F., Wahlefeld, A.-W. and Persijn, J.-P., 1974. New substrates for measuring γ-glutamyl transpeptidase activity.Zeitschrift für Klinische Chemie und Klinische Biochemie,12, 228
Wahlefeld, A.W., Herz, G. and Bernt, E., 1972. Modification of the Malloy-Evelyn method for a simple, reliable determination of total bilirubin in serum.Scandinavian Journal of Clinical and Laboratory Investigation,29 (Supplement 126), Abstract 11.12
White, A., Handler, P. and Smith, E.L., 1968. Enzymes. I. Nature and classification. In:Principles of Biochemistry, 4th edn, (McGraw-Hill, New York), 208–222
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FlÅøyen, A., Smith, B.L. Parenchymal injury and biliary obstruction in relation to photosensitization in sporidesmin-intoxicated lambs. Vet Res Commun 16, 337–344 (1992). https://doi.org/10.1007/BF01839183
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DOI: https://doi.org/10.1007/BF01839183