Abstract
Differences in antigenicity between human and canine C-reactive proteins were investigated by Western blotting analysis. It was confirmed that several commercial anti-human CRP sera reacted with canine CRP. However, 34 anti-canine CRP sera prepared by immunization of rabbits and goats with canine CRP all reacted with canine CRP but not with human CRP in either immunoelectrophoresis or Western blotting.
Immunization with human CRP produced a cross-reacting antibody that reacted with canine CRP. Conversely, immunization with canine CRP did not produce a cross-reacting antibody that reacted with human CRP. These findings may be interpreted as showing that, while canine and human CRPs do not share common antigenicity, they do contain structurally similar antigenic determinants.
Similar content being viewed by others
Abbreviations
- CRP:
-
C-reactive protein
- PBS:
-
phosphate-buffered saline
- SDS-PAGE:
-
sodium dodecyl sulphate-polyacrylamide gel electrophoresis
References
Blake, M.S., Johnston, K.H., Russell-Jones, G.J. and Gotschlich, E.C., 1984. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.Analytical Biochemistry,136, 175–179
Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical Biochemistry,72, 248–254
Caspi, D., Baltz, M., Snel, F., Gruys, E., Niv, D., Batt, R.M., Munn, E.A., Buttress, N. and Pepys, M.B., 1984. Isolation and characterization of C-reactive protein from the dog.Immunology,53, 307–313
Caspi, D., Snel, F.W.J.J., Batt, R.M., Bennett, D., Rutteman, G.R., Hartman, E.G., Baltz, M.L., Gruys, E. and Pepys, M.B., 1987. C-reactive protein in dogs.American Journal of Veterinary Research,48, 919–921
Conner, J.G., Eckersall, P.D., Ferguson, J. and Douglas, T.A., 1988. Acute phase response in the dog following surgical trauma.Research in Veterinary Science,45, 107–110
Dillman, R.C. and Coles, E.H., 1966. A canine serum fraction analogous to human C-reactive protein.American Journal of Veterinary Research,27, 1769–1775
Eckersall, P.D., Conner, J.G. and Parton, H., 1989. An enzyme-linked immunosorbent assay for canine C-reactive protein.Veterinary Record,124, 490–491
Eckersall, P.D., Conner, J.G. and Harvie, J., 1991. An immunoturbidimetric assay for canine C-reactive protein.Veterinary Research Communications,15, 17–24
Gotschlich, E., 1962. Occurrence of a substance analogous to C-reactive protein in acute phase dog sera.Federation Proceedings,21, 14
Goudswaard, J., Van der Donk, J.A., Noordzij, A., Van Dam, R.H. and Vaerman, J-P., 1978. Protein A reactivity of various mammalian immunoglobulins.Scandinavian Journal of Immunology,8, 21–28
Iwaki, T., 1990. A rapid diagnosis method of infectious disease (C reactive protein: CRP).Companion Animal Practice,25, 69–73 (in Japanese)
Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature,227, 680–685
Mallya, R.K., de Beer, F.C., Berry, H., Hamilton, E.D.B., Mace, B.E.W. and Pepys, M.B., 1982. Correlation of clinical parameters of disease activity in rheumatoid arthritis with serum concentration of C-reactive protein and erythrocyte sedimentation rate.Journal of Rheumatology,9, 224–228
Maudsley, S. and Pepys, M.B., 1987. Immunochemical cross-reactions between pentraxins of different species.Immunology,62, 17–22
Maury, C.P.J., 1985. Comparative study of serum amyloid A protein and C-reactive protein in disease.Clinical Science,68, 233–238
McFarlane, H., Ngu, V.A., Udeozo, I.O.K., Osunkoya, B.O., Luzzatto, L. and Mottram, F.C., 1967. Some acute phase proteins in Burkitt lymphoma in Nigerians.Clinica Chimica Acta,17, 325–329
Ndung'u, J.M., Eckersall, P.D. and Jennings, F.W., 1991. Evaluation of the concentration of acute phase proteins in dogs infected withTrypanosoma brucei.Acta Tropica,49, 77–85
Pepys, M.B. and Baltz, M.L., 1983. Acute-phase proteins with special reference to C-reactive protein and related proteins (pentaxins) and serum amyloid A protein.Advances in Immunology,34, 141–212
Riley, R.F. and Coleman, M.K., 1970. Isolation of C-reactive proteins of man, monkey, rabbit and dog by affinity chromatography on phosphorylated cellulose.Clinica Chimica Acta,30, 483–496
Riley, R.F. and Zontine, W., 1972. Further observations on the properties of dog C-reactive protein and the C-reactive protein response in the dog.Journal of Laboratory and Clinical Medicine,80, 698–703
Towbin, H., Staehelin, T. and Gordon, J., 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications.Proceedings of the National Academy of Sciences of the USA,76, 4350–4354
Yamamoto, S., Tagata, K., Nagahata, H., Ishikawa, Y., Morimatsu, M. and Naiki, M., 1992. Isolation of canine C-reactive protein and characterization of its properties.Veterinary Immunology and Immunopathology,30, 329–339
Yamamoto, S., Abe, N., Santsuka, H., Shida, T., Kishida, K., Kuwajima, S., Yamada, M., Morimatsu, M. and Naiki, M., 1993a. Efficient preparation of monospecific anti-canine CRP serum and purification of canine CRP by affinity chromatography.Veterinary Immunology and Immunopathology,36, 293–301
Yamamoto, S., Miyaji, S., Tagata, K., Shida, T., Fujise, H., Mukawa, K., Furukawa, E., Nagae, T. and Naiki, M., 1993b. Serum levels of C-reactive protein in dogs and its simple technique for measurement.Journal of the Japan Veterinary Medical Association, in press (in Japanese with English summary)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yamamoto, S., Miyaji, S., Abe, N. et al. Canine C-reactive protein (CRP) does not share common antigenicity with human CRP. Vet Res Commun 17, 259–266 (1993). https://doi.org/10.1007/BF01839216
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01839216