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A Mercury buffer for toxicity experiments with green algae

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Abstract

Mercury(II) toxicity experiments with green algae are complicated by the fast reduction and evaporation of Hg. A Hg buffer system is described, which considerably stabilizes the Hg(II) concentration in test solutions. The Hg buffer consists of mercury(II) chloride and N-methyliminodiacetic acid (MIDA). Dissociation of Hg-MIDA complex compensates for loss of Hg. With this system experiments were performed with Hg(II) concentrations between 0.02 and 2.0 mg I−1 at temperatures between 15° and 30°C. No effect of MIDA on the growth of the green alga Scenedesmus acutus was detected.

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Authors and Affiliations

  1. Laboratory of General and Technical Biology, Delft University of Technology, Julianalaan 67, -2208, Delft, the Netherlands

    J. Huisman & H. J. G. Ten Hoopen

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  1. J. Huisman
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  2. H. J. G. Ten Hoopen
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Huisman, J., Ten Hoopen, H.J.G. A Mercury buffer for toxicity experiments with green algae. Water Air Soil Pollut 10, 325–333 (1978). https://doi.org/10.1007/BF00285060

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  • DOI: https://doi.org/10.1007/BF00285060

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