Abstract
The movement of bacteria and bacteriophages into and through an alluvial gravel aquifer was investigated at a bordered strip effluent irrigation scheme near Christchurch, New Zealand. Irrigation of one set of strips resulted in the contamination, by faecal coliform bacteria, and somatic and F-RNA coliphages, of two bores, approximately 60 m and 445 m downstream of the centre of the strips. F-RNA coliphages showed the greatest attenuation between the soil surface and the first bore, and faecal coliforms the least. Estimates of percolation times through the 13 m vadoze zone (based on times to peak concentration in the groundwater) ranged from 1.6 to 10.5 hr, with travel times for the bacteriophages being 1.4–3.4 times longer than for the bacteria. Injection of oxidation pond effluent containing rhodamine WT dye into the first bore resulted in contamination of the second bore (385 m downstream) by the dye, F-RNA coliphages, and faecal coliforms. In a second experiment, injection (into the same bore) of a mixture of phage MS–2, the bacterial tracer Escherichia coli J6–2, and rhodamine WT dye, produced a similar result in the downstream bore and in a newly-installed bore, 401 m downstream. In both injection experiments, the phages exhibited the shortest times to peak concentrations in the downstream bore(s), followed by the bacteria, and then the dye. Attenuation of the bacteria and phages was similar, but the microbes exhibited 100-fold greater reduction than the dye. Flow direction and longitudinal dispersivity were determined in a preliminary analysis using an idealised 2-D dispersion model. This information, and other measured and reported data, were then used as inputs in a 3-D dispersion model. The predicted concentration curves were matched to the observed curves by trial and error adjustment of the decay constant (λ). The best curve fits were obtained with λ values higher than those reported elsewhere. It is suggested that many of the reported microbial decay values underestimate microbial reductions in groundwater because they do not account for other removal mechanisms, such as filtration, sedimentation and irreversible adsorption.
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References
Abu-Ashour, J., Joy, D. M., Lee, H., Whitely, H. R. and Zelin, S.: 1994, Water, Air, and Soil Pollut. 75, 141.
Adams, M. H.: 1959, Bacteriophages, Interscience Publishers Inc., New York. p. 592.
American Public Health Association: 1992, Standard Methods for the Examination of Water and Wastewater, 18th Edition. American Public Health Association, American Water Works Association, Water Pollution Control Federation. p. 949.
Ayrey, R. B. and Noonan, M. J.: 198¿, ‘The effect of land use on groundwater quality in central Canterbury’, in M. J. Noonan (ed.), Microbiology and Water Quality, Proceedings, Joint Conference N.Z. Committee on Water Pollution Research and N.Z. Microbiological Society. Technical Publication No. ¿, Department of Agricultural Microbiology, Lincoln College, Canterbury, New Zealand, pp. 69–78.
Bales, R. C., Gerba, C. P., Grondin, G. H. and Jensen, S. L.: 1989, Appl. Environ. Microbiol. 55, 2061.
Bales, R. C., Hinkle, S. R., Kroeger, T. W. and Stocking, K.: 1991, Environ. Sci. Technol. 25, 2088.
Beven, K. and Germann, P.: 1982, Water Resour. Res. 18, 1¿11.
Bitton, G. and Farrah, S. R.: 1986, Rev. Internat. des Sci. de l'Eau. 2, ¿1.
Bitton, G., Farrah, S. R., Ruskin, R. H., Butner, J. and Chou, Y. G.: 198¿, Ground Water 21, 405.
Canterbury Regional Council: 1990, General Authorisation for Septic Tank Effluent Disposal. Canterbury Regional Council, Christchurch, New Zealand, p. 9.
Champ, D. R. and Schroeter, J.: 1988, ‘Bacterial Transport in Fractured Rock - A field scale tracer test in the Chalk River nuclear laboratories’, in B. H. Olson and D. Jenkins (eds.), Proceedings of the International Conference on Water and Wastewater Microbiology, Newport Beach, California, 8- 11 February, 1988, pp. 81–87.
Childs, C. W., Searle, P. L. and Wells, N.: 1977, Infiltration through Soil as a Tertiary Treatment of Effluent, New Zealand Soil Bureau Scientific Report No. 29, Department of Scientific and Industrial Research, Wellington. p. 28.
Corapcioglu, M. Y. and Haridas, A.: 1984, J. Hydrol. 72, 149.
Corapcioglu, M. Y. and Haridas, A.: 1985, Adv. Water Resour. 8, 188.
Craun, G. F.: 1991, Wat. Sci. Tech. bf 24, 17.
Dickenson, R. A.: 1991, ‘Problems with using existing transport models to describe microbial transport in porous media’, in C. J. Hurst (ed.), Modeling the Environmental Fate of Microorganisms, American Society for Microbiology, pp. 21–47.
Domenico, P. A. and Schwartz, F. W.: 1990, Physical and Chemical Hydrogeology, John Wiley & Sons, New York. p. 807.
Gerba, C. P. and Bitton, G.: 1984, ‘Microbial pollutants. Their survival and transport pattern to groundwater’, in G. Bitton and C. P. Gerba (eds.), Groundwater Pollution Microbiology, John Wiley & Sons, Inc., New York, pp. 65–88.
Gerba, C. P. and Goyal, S. M.: 1985, ‘Pathogen removal from wastewater during groundwater recharge’, in T. Asano (ed.), Artificial Recharge of Groundwater, Butterworth Publishers, pp. 28¿–¿17.
Gerba, C. P., Goyal, S. M., Cech, I. and Bogdan, G. F.: 1982, Environ. Sci. Technol. 15, 940.
Gerba, C. P., Yates, M. V. and Yates, S. R.: 1991, ‘Quantitation of factors controlling viral and bacterial transport in the subsurface’, in C. J. Hurst (ed.), Modelling the Environmental Fate of Microorganisms, American Society for Microbiology, pp. 77–88.
Germann, P. F., Smith, M. S. and Thomas, G. W.: 1987, Wat. Resour. Res. 2¿, 1281.
Guy, E. M. and Small, J. A.: 1977, N. Z. J. Agric. Res. 20, 1¿.
Guy, E. M and Visser, T. A.: 1979, N. Z. J. Agric. Res. 22, ¿41.
Harvey, R. W.: 1991, ‘Parameters involved in modeling movement of bacteria in groundwater’, in C. J. Hurst (ed.), Modeling the Environmental Fate of Microorganisms, American Society for Microbiology, pp. 89–114.
Harvey, R. W., George, L. H., Smith, R. L. and LeBlanc D. R.: 1989, Environ. Sci. Technol. 2¿, 51.
Harvey, R. W. and Garabedian, S. P.: 1991, Environ. Sci. Technol. 25, 178.
Havelaar, A. H. and Hogeboom, W. M.: 1984, J. Appl. Bacteriol. 56, 4¿9.
Havelaar, A. H., van Olphen, M. and Drost, Y.: 199¿, Appl. Environ. Microbiol. 59, 2956.
Hopper, M. J.: 197¿, ‘Harwell subroutine library: a catalogue of subroutines’, United Kingdom Atomic Energy Authority, Report 7477, Harwell, p. 118.
IAWPRC.: 1991, Water Res. 25, 529.
Kear, B. S., Gibbs, H. S. and Miller, R. B.: 1967, ‘Soils of the Downs and Plains, Canterbury and North Otago’, N. Z. Soil Bureau Bulletin 14, p. ¿5.
Keswick, B. H. and Gerba, C. P.: 1980, Environ. Sci. Technol. 14, 1290.
Martin, G. N. and Noonan, M. J.: 1977, Effects of Domestic Wastewater Disposal by Land Irrigation on Groundwater Quality of the Central Canterbury Plains. Water and Soil Technical Publication No. 7, Ministry of Works and Development, Wellington, New Zealand, p. 25.
Marzouk, Y., Goyal, S. M. and Gerba, C. P.: 1979, Ground Water 17, 487.
Matthess, G., Pekdeger, A. and Schroeter, J.: 1988, J. Contam. Hydrol. 2, 171.
McFeters, G. A., Bissonnete, G. K., Jeseki, J. J., Thomson, C. A. and Stuart, D. G.: 1974, Appl. Environ. Microbiol. 27, 82¿.
Moore, B. E., Sagik, B. P. and Sorber, C. A.: 1981, J. Water Poll. Contr. Fed. 5¿, 10.
Noonan, M. J. and McNabb, J. F.: 1979, ‘Contamination of Canterbury groundwater by viruses’, in M. J. Noonan (ed.), Groundwater. The Quality and Movement of Groundwater in Alluvial Aquifers of New Zealand, Department of Agricultural Microbiology Technical Publication No. 2, Lincoln University, Canterbury, New Zealand, pp. 195–201.
Park, N., Blandford, T. N. and Huyakorn, P. S.: 1992, VIRALT: A Modular Semi-Analytical and Numerical Model for Simulating Viral Transport in Groundwater, Prepared by HydroGeologic Inc. for USEPA. International Ground Water Modelling Centre, Colorado School of Mines, p. 74.
Pekdeger, A. and Matthess, G.: 198¿, Environmental Geology 5, 49.
Peterson, T. C. and Ward, R. C.: 1989, Water Resources Bull. 25, ¿49.
Rao, V. C. and Melnick, J. L.: 1986, Environmental Virology, American Society for Microbiology, Washington, D.C., USA, 88 pp.
Sinton, L.W.: 1980a, Investigations into the Use of the Bacterial Species Bacillus stearothermophilus and Escherichia coli (H2S+) as Tracers of Groundwater Movement, Water and Soil Technical Publication No. 17, MWD, Wellington, p. 24.
Sinton, L. W.: 1980b, J. Hydrol. (NZ) 19, 119.
Sinton, L. W.: 1982, N. Z. J. Marine Freshwater Res. 16, ¿17.
Sinton, L. W.: 1984, Hydrobiologia 119, 161.
Sinton, L. W.: 1986, Water, Air, and Soil Pollut. 28, 407.
Sinton, L. W. and Close, M. E.: 198¿, Groundwater Tracing Experiments, Publication No. 2 of the Hydrology Centre, Ministry of Works and Development, Christchurch, New Zealand, p. ¿8.
Sinton, L. W., Finlay, R. K. and Reid A. J.: 1996, J. Microbiol. Methods 25, 257.
Slade, J. S.: 1985, Viruses and bacteria in a chalk well, Water Science and Technology 17, 111–125.
Smart, P. L. and Laidlaw, I. M. S.: 1977, Water Resources Res. 1¿, 15.
Smith, M. S., Thomas, G. W., White, E. E. and Retonga, D.: 1985, J. Environ. Qual. 14, 87.
Sobsey, M. D., Shields, P. A., Hauchman, F. H., Hazard, R. L. and Caton, L. W.: 1986, Water. Sci. Technol. 18, 97.
Suggate, R. P.: 197¿, Geological map of New Zealand, Sheet 21, Christchurch (2nd edition). N Z Department of Scientific and Industrial Research, Wellington.
Thorpe, H. R., Burden, R. J. and Scott, D. M.: 1982, Potential for Contamination of the Heretaunga Plains Aquifers, Water and Soil Technical Publication No. 24, Ministry of Works and Development, Wellington, New Zealand, p. 149.
Tim, U. S. and Mostaghimi, S.: 1991, Ground Water 29, 251.
Vaughn, J. M. and Landry, E. F.: 198¿, ‘Viruses in soils and groundwaters’, in G. Berg (ed.), Viral Pollution of the Environment. CRC Press. pp. 16¿–210.
Vilker, V. L. and Burge, W. D.: 1980, Water Res. 14, 78¿.
Vilker, V. L.: 1981, ‘Simulating virus movement in soils’, in I. K. Iskandar (ed.), Modelling Wastewater Renovation: Land Treatment. John Wiley & Sons, New York, pp. 22¿–25¿.
Westwood, J. C. N. and Sattar, S. A.: 1976, ‘The minimal infective dose’, in G. Berg, H. L. Bodily, E. H. Lennette, J. L. Melnick and T. G. Metcalf (eds.), Viruses in Water. American Public Health Association, Washington, D. C. pp. 61–69.
Wilson, J. L. and Miller, P. J.: 1978, J. Hydraulics Div., Am. Soc. Civil Eng. 104, 50¿.
Wilson, L. G., Gerba, C. P., Bolton, M. W. and Rose, J. B. P: 1984, ‘Subsurface transport of urban runoff pollutants’, in N. N. Durham and A. E. Redelfs (eds.), Proc. of the Second International Conference on Groundwater Quality Research, University Printing Services, Oklahoma State University, Stillwater, pp. 158–160.
Wood, W. W. and Erlich, G. G.: 1978, Ground Water 16, ¿98.
Yahya, M. T., Galsomies, L., Gerba, C. P. and Bales, R. C.: 199¿, Water. Sci. Technol. 27, 409.
Yates, M. V., Gerba, C. P. and Kelly, L. M.: 1985, Appl. Environ. Microbiol. 49, 778.
Yates, M. V. and Yates, S. R.: 1987, Water Res. 21, 1119.
Yates, M. V., Yates, S. R., Wagner, J. and Gerba, C. P.: 1987, J. Contam. Hydrol. 1, ¿29.
Yeh, G. T.: 1992, AT12¿D - Analytical Transient One-, Two-and Three-Dimensional Simulation of Waste Transport in the Aquifer System, International Ground Water Modelling Centre, Colorado School of Mines, 79 pp.
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SINTON, L.W., FINLAY, R.K., PANG, L. et al. TRANSPORT OF BACTERIA AND BACTERIOPHAGES IN IRRIGATED EFFLUENT INTO AND THROUGH AN ALLUVIAL GRAVEL AQUIFER. Water, Air, & Soil Pollution 98, 17–42 (1997). https://doi.org/10.1023/A:1026492110757
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DOI: https://doi.org/10.1023/A:1026492110757