Abstract
A crude outer-membrane protein (OMP) preparation from a strain of Bacteroides fragilis, grown in supplemented brain-heart infusion broth, was tested for its protective effect against subcutaneous infection in mice. Immunization with six doses, each of 100, 150 or 200 μg OMP preparation, gave some protection: abscesses completely disappeared 15 to 22 days after immunization. In non-immunized animals and animals immunized with doses of 10, 20, 40 or 80 μg each, well demarcated abscesses were seen beyond day 22 post-immunization. Although crude OMP elicited good antibody response, with maximum titres on day 4 post-immunization, high titres could not be associated with healing of the abscesses.
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Khanna, T., Panigrahi, D., Ganguly, N.K. et al. Immunization with outer-membrane protein(s) of Bacteroides fragilis: an experimental study in mice. World J Microbiol Biotechnol 10, 645–647 (1994). https://doi.org/10.1007/BF00327951
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DOI: https://doi.org/10.1007/BF00327951