Abstract
The effects of experimental factors on protoplast formation of Prototheca zopfii Kru¨ger in 0.85 m NaCl using Macerozyme R-200 were studied based on a fractional factorial experimental design. The rate of protoplast formation was mainly affected by the incubation temperature and the age of algal cells. The optimal condition for the maximum protoplast yield was determined based on a response surface model. These were: mid-logarithmic phase cells and Macerozyme concentration of 4% at a temperature of 35°C.
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Suzuki, T., Yamaguchi, T. & Ishida, M. Effects of some factors on protoplast formation of a microalga, Prototheca zopfii. World Journal of Microbiology and Biotechnology 13, 355–356 (1997). https://doi.org/10.1023/A:1018555628895
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DOI: https://doi.org/10.1023/A:1018555628895