Abstract
Scleroglucan production by Sclerotium rolfsii ATCC 201126 has been studied using nitrate or ammonium as nitrogen sources at several concentrations. In all the experiments carried out, both growth and production were modelled by an unstructured kinetic model using logistic and Luedeking–Piret equations for describing growth and production, respectively. The kinetic parameters for growth (μ and YXN) and for production (α and β) were obtained by fitting the data to the model using the single-response non-linear regression technique by means of the algorithm of Marquardt coupled to a fourth-order Runge–Kutta algorithm. Biomass and scleroglucan production were higher when nitrate was the nitrogen source. Rheological properties of scleroglucan produced using nitrate as nitrogen source were studied and rheological parameters calculated, revealing similarity between this biopolymer and commercial scleroglucan.
Similar content being viewed by others
References
Bongenaar, J.J.T.M., Kossen, N.W.F., Metz, B. & Meijboom, F.W. 1973 A method for characterizing the rheological properties of viscous fermentation broths. Biotechnology & Bioengineering 15, 201–206.
Brigand, G. 1993 Scleroglucan. In Industrial Gums. Polysac-charides and their derivatives, eds. R.L. Whistler, & J.N. BeMiller, 3rd edn. Academic Press Inc. SanDiego, USA. ISBN 0–12–746253–8.
Deed, A. & Seviour, R. 1989 Formation and regeneration of protoplasts of Sclerotium glucanicum. Applied Microbiology and Biotechnology 31, 259–264.
Fariña, J.I., Siñeriz, F., Molina, O.E. & Perotti, N.I. 1996 Low-cost method for the preservation of Sclerotium rolfsii Proimi F-6656: Inoculum standardization and its use in scleroglucan production. Biotechnology Techniques 10, 705–708.
García-Ochoa, F. & Santos, V.E. 1994 Revisión. Cine tica de transformaciones usando microorganismos I: Modelos cine ticos no estructurados. Anales de QuõÂmica 90, 7–17.
Griffith, W.L. & Compere, A.L. 1978 Production of a high viscosity glucan by Sclerotium rolfsii ATCC 15206. Devel-opments in Industrial Microbiology 19, 609–617.
Halleck, F.E. 1967 Polysaccharides and methods for produc-tion thereof. US Patent, 3,301,848.
Marquardt, D.W. 1963 An algorithm for least-squares estima-tion of non-linear parameters. Journal Society for Industrial and Applied Mathematics 11, 431–441.
Pilz, F., Auling, G., Stephan, D., Rau, U. & Wagner, F. 1991 A high-affinity Zn2. uptake system controls growth and bio-synthesis of an extracellular, branched b-1,3–b-1,6–glucan in Sclerotium rolfsii ATCC 15205. Experimental Mycology 15, 181–192.
Taurhesia, S. & McNeil, B. 1994a Production of scleroglucan by Sclerotium glucanicum in batch and supplemented batch cultures. Enzyme and Microbial Technology 16, 223–228.
Taurhesia, S. & McNeil, B. 1994b Physicochemical factors af-fecting the formation of the biological response modifier scleroglucan. Journal of Chemical Technology and Biotech-nology 59, 157–163.
Wang, Y. & McNeil, B. 1996 Scleroglucan. Critical Reviews in Biotechnology 16, 185–215.
Yanaki, T., Kojima, T. & Norisuye, T. 1981 Triple helix of scleroglucan in dilute aqueous sodium hydroxide. Polymer Journal 13, 1135–1143.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Fariña, J., Santos, V., Perotti, N. et al. Influence of the nitrogen source on the production and rheological properties of scleroglucan produced by Sclerotium rolfsii ATCC 201126. World Journal of Microbiology and Biotechnology 15, 309–316 (1999). https://doi.org/10.1023/A:1008999001451
Issue Date:
DOI: https://doi.org/10.1023/A:1008999001451