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Diagnosis of melioidosis by means of an ELISA detecting antibodies toPseudomonas pseudomallei exotoxin. Preliminary assay evaluation

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Abstract

An ELISA system for the detection of human antibodies toPseudomonas pseudomallei exotoxin is described. Analysis of control sera and sera from patients with culture-positive melioidosis showed that both IgG and IgM antibodies to exotoxin were detectable in patients with melioidosis. This implies that the exotoxin is producedin vivo byP. pseudomallei, and may be involved in the pathogenesis of melioidosis. Furthermore, these rapid and simple assays may prove to be useful for the serodiagnosis of the disease.

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Author notes

  1. D. A. B. Dance

    Present address: Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, Keppel Street, WC1E 7HT, London, UK

Authors and Affiliations

  1. Faculty of Science and Innovation, The North East Wales Institute, CH5 4BR, Deeside, Clwyd, UK

    C. J. Smith

  2. Wellcome-Mahidol University-Oxford Tropical Medicine Research Programme, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, 10400, Bangkok, Thailand

    D. A. B. Dance & N. J. White

  3. Nuffield Department of Clinical Medicine, John Radcliffe Hospital, OX3 9DU, Headington, Oxford, UK

    D. A. B. Dance & N. J. White

  4. Department of Medicine, Sappasitprasong Hospital, Ubon Ratchatani, Thailand

    W. Chaowagul

Authors
  1. C. J. Smith
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  2. M. Jones
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  3. D. A. B. Dance
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  4. W. Chaowagul
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  5. N. J. White
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Smith, C.J., Jones, M., Dance, D.A.B. et al. Diagnosis of melioidosis by means of an ELISA detecting antibodies toPseudomonas pseudomallei exotoxin. Preliminary assay evaluation. World J Microbiol Biotechnol 7, 29–36 (1991). https://doi.org/10.1007/BF02310916

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  • DOI: https://doi.org/10.1007/BF02310916

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