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Improvement of the alcoholic fermentation of grape juice with mixed cultures of Saccharomyces cerevisiae wild strains. Negative effect of Kloeckera apiculata

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Abstract

Four assays were designed to study the fermentative behaviour of two selected wild strains of Saccharomyces cerevislae when inoculated in sterilized grape juice either alone or in association. In addition, a wild strain of Kloeckera apiculata was employed for studying and characterizing the influence of this micro-organism on the fermentations when associated with Sacch. cerevisiae. Fermentation was improved when the two strains of Sacch. cerevisiae were grown together since the residual sugar fell to 0.12% and ethanol increased up to more than 11% (v/v). In this case, a proliferation of the two strains with successive exponential phases was observed. On the other hand, when Kloeckera apiculata was incorporated into the fermentations, these were incomplete with a residual sugar level of 1.64% and a final ethanol concentration of 6.4%.

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References

  • Amerine M.A. & Kunkee R.E. 1968 Microbiology of winemaking. Annual Review of Microbiology, 22, 323–356.

    Google Scholar 

  • Benda I. 1982 Wine and brandy. In Prescott and Dunn's Industrial Microbiology 4th edn, ed. Reed G.H. pp. 293–402. Westport, CT: AVI Technical Books Inc.

    Google Scholar 

  • Bertrand A., Marly-Brugerole C. & Sarre C. 1978 Influence du debourbage des moûts et du sulfitage sur les teneurs en substances volatils des vins et des eaux de vie. Connaissance Vigne Vin 12, 35–48.

    Google Scholar 

  • Calleja G.B. & Johnson F. 1977 A comparison of quantitative methods for measuring yeast flocculation. Canadian Journal of Microbiology 23, 68–74.

    Google Scholar 

  • Cansado J., Longo E., Agrelo D. & Villa T.G. 1989 Levaduras asociadas a procesos de fermentación espontánea en vinos de Ribeiro. Análisis del homo/heterotalismo y sistema killer de las cepas de Sacch. cerevisiae. Microbiología SEM 5, 79–88.

    Google Scholar 

  • Fleet G.H., Lafon-Lafourcade S. & Ribéreau-Gayon P. 1984 Evolution of yeasts and lactic acid bacteria during fermentation and storage of Bordeaux wines. Applied and Environmental Microbiology 48, 1034–1038.

    Google Scholar 

  • Geneix C., Lafon-Lafourcade S. & Ribéreau-Gayon P. 1983 Effect des acids gras sur la viabilité des populations de Saccharomyces cerevisiae. C. R. Academie de Science de Paris 296D, Serie III, 943–947.

    Google Scholar 

  • Heard G.M. & Fleet G.H. 1985 Growth of natural yeast flora during the fermentation of inoculated wines. Applied and Environmental Microbiology 50, 727–728.

    Google Scholar 

  • Lafon-Lafourcade S. & Ribéreau-Gayon P. 1984 In Progress in Industrial Microbiology; modern application of traditional biotechnologies, ed. Bushell M.E., vol. 19, pp. 1–45. Oxford: Elsevier Publishing Co.

    Google Scholar 

  • Rankine B.C. 1968 The importance of yeasts in determining the composition and quality of wines. Vitis 7, 22–49.

    Google Scholar 

  • Reed G. & Nagodawithana W. 1988 Technology of yeast usage in winemaking. American Journal of Enology and Viticulture 39, 83–90.

    Google Scholar 

  • Ribéreau-Gayon P., Peynaud E. & Sudraud P. 1978 Sciences et techniques du vin (Tome II). Paris: Dunod.

    Google Scholar 

  • Sapis-Domercq S. 1958 Comportement des levures apiculées au cours de la vinification. Connaissance Vigne Vin 1, 379–392.

    Google Scholar 

  • Seki T., Choi E. & Dewey R. 1985 Construction of killer yeast strain. Applied and Environmental Microbiology 49, 1211–1215.

    Google Scholar 

  • Shimazu Y. & Watanabe M. 1981 Effects of yeast strains and environmental conditions on formation of organic acids in must during fermentation. Journal of Fermentation Technology 59, 27–32.

    Google Scholar 

  • Sikovec S. 1976 The selection of indigenous yeasts for winemaking. Annals of Microbiology and Enzymology 26, 69–73.

    Google Scholar 

  • Soufleros E. & Bertrand A. 1979 Role de la souche de levure dans la production de substances voltiles au cours de la fermentation du jus de raisin. Connaissance Vigne Vin 13, 181–192.

    Google Scholar 

Download references

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The authors are with the Cátedra de Microbiología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, Spain. T.G. Villa is the corresponding author.

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Velázquez, J.B., Longo, E., Sieiro, C. et al. Improvement of the alcoholic fermentation of grape juice with mixed cultures of Saccharomyces cerevisiae wild strains. Negative effect of Kloeckera apiculata . World Journal of Microbiology and Biotechnology 7, 485–489 (1991). https://doi.org/10.1007/BF00303374

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  • DOI: https://doi.org/10.1007/BF00303374

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